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Antimicrobial susceptibility and molecular typing of Pasteurella multocida isolated from six provinces in China

Ling-Cong Kong1,2, Zi Wang1, Yi-Ming Wang1

  • 1Department of Basic Veterinary Medicine, College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, Jilin, China.

Tropical Animal Health and Production
|December 12, 2018
PubMed

Insights

Multidrug-resistant Pasteurella multocida strains causing bovine respiratory disease (BRD) are spreading across China. These resistant bacteria, found in multiple provinces, also carry mobile genetic elements conferring further resistance.

Area of Science:

  • Veterinary Microbiology
  • Antimicrobial Resistance
  • Genomics

Background:

  • Pasteurella multocida is a key pathogen responsible for bovine respiratory disease (BRD).
  • Understanding antimicrobial resistance patterns is crucial for managing BRD in cattle populations.
  • Previous studies indicate varying resistance profiles, necessitating regional investigation.

Purpose of the Study:

  • To determine the antimicrobial susceptibility of Pasteurella multocida isolates from various Chinese provinces.
  • To investigate the genetic relatedness and identify resistance mechanisms in multidrug-resistant strains.
  • To analyze the prevalence of class I integrons in these isolates.

Main Methods:

  • Antimicrobial susceptibility testing was performed on P. multocida isolates.
  • Pulsed-field gel electrophoresis (PFGE) was used to assess genetic diversity and clonal spread.
  • Whole-genome sequencing was conducted on a representative multidrug-resistant strain.
  • Class I integron detection was performed.

Main Results:

  • Isolates showed significant resistance to amikacin, sulfamethoxazole, sulfachloropyridazinesodium, macrolides, and fluoroquinolones.
  • PFGE analysis revealed clonal spread of multidrug-resistant P. multocida across different provinces.
  • All analyzed isolates harbored class I integrons, suggesting a role in resistance dissemination.

Conclusions:

  • Multidrug-resistant P. multocida strains are prevalent and clonally spreading in China, posing a significant threat to cattle health.
  • The presence of class I integrons in all isolates highlights their importance in the acquisition and spread of antimicrobial resistance.
  • Effective control strategies for BRD must address the challenge of multidrug-resistant P. multocida.
Keywords:
Minimal inhibitory concentrationMolecular typingPasteurella multocida

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