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Updated: Feb 1, 2026

Hepatic Progenitor Specification from Pluripotent Stem Cells using a Defined Differentiation System
Published on: May 10, 2020
A Rodent Model for Cell Transplantation of Hepatic Progenitor Cells
Sei Kakinuma1,2, Akihide Kamiya3
1Department of Gastroenterology and Hepatology, Tokyo Medical and Dental University, Tokyo, Japan. skakinuma.gast@tmd.ac.jp.
Abstract:
Hepatic progenitor cells are defined as cells exhibiting potency for active proliferation and capacity for bipotential differentiation into hepatocytes and cholangiocytes. To prove the capacity of target cells for terminal differentiation and reconstitution of organs, cell transplantation models have been widely used in previous studies, including those involving the liver. Here we describe a protocol for transplantation of hepatic progenitor cells using retrorsine pretreatment and partial hepatectomy. This transplantation assay reveals the potential for reconstitution of hepatocytes in recipient livers by primary hepatic progenitor cells. Donor cells are detected as a colony composed of 5-10 mature hepatocytes.
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