Related Experiment Video

Updated: Feb 1, 2026

A Sensitive Method to Quantify Senescent Cancer Cells
09:18

A Sensitive Method to Quantify Senescent Cancer Cells

Published on: August 2, 2013

21.3K

DUSP22 promotes senescence of HS-1 skin cancer cells through triggering MAPK signaling pathway

X-D Zhao1, C Huang, R-X Wang

  • 1Department of Dermatology, The Fifth Affiliated Hospital of Harbin Medical University, Daqing, Heilongjiang, China. fangduanzibeiz@163.com.

European Review for Medical and Pharmacological Sciences
|December 12, 2018
PubMed
Abstract

Insights

Dual-specificity protein phosphatase 22 (DUSP22) promotes skin cancer cell aging by activating the MAPK pathway. This finding offers a potential new strategy for treating skin cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Biology

Background:

  • Skin cancer poses a significant public health risk.
  • Dual-specificity protein phosphatase 22 (DUSP22) plays a role in cell growth and proliferation.
  • Understanding DUSP22's role in skin cancer aging is crucial for developing new therapies.

Purpose of the Study:

  • To investigate the regulatory role of DUSP22 in skin cancer cell aging.
  • To elucidate the molecular mechanisms underlying DUSP22-induced aging.
  • To explore the potential therapeutic applications of targeting DUSP22 in skin cancer.

Main Methods:

  • HS-1 skin cancer cells were transfected with DUSP22 or DUSP22 siRNA.
  • Western blot and RT-PCR were used to assess DUSP22 expression and MAPK pathway activation.
  • Levels of aging proteins, including P53 and P21, were evaluated.

Main Results:

  • DUSP22 overexpression enhanced DUSP22 expression and activated the MAPK pathway, inducing cell aging.
  • DUSP22 knockdown suppressed DUSP22 expression, inhibited the MAPK pathway, and reduced aging proteins P53 and P21.
  • DUSP22 was found to be downregulated in HS-1 cells, correlating positively with P53 expression.

Conclusions:

  • DUSP22 facilitates skin cancer cell aging through MAPK pathway activation.
  • Targeting DUSP22 may represent a novel therapeutic strategy for skin cancer treatment.

More Related Videos

Far-Red Fluorescent Senescence-Associated β-Galactosidase Probe for Identification and Enrichment of Senescent Tumor Cells by Flow Cytometry14:01

Far-Red Fluorescent Senescence-Associated β-Galactosidase Probe for Identification and Enrichment of Senescent Tumor Cells by Flow Cytometry

Published on: September 13, 2022

5.8K
Drug-Induced Senescence in Liver Cells Promotes M2 Macrophage Polarization: Implications for Tyrosine Kinase Inhibitor-Associated Hepatotoxicity09:32

Drug-Induced Senescence in Liver Cells Promotes M2 Macrophage Polarization: Implications for Tyrosine Kinase Inhibitor-Associated Hepatotoxicity

Published on: October 17, 2025

485

Related Concept Videos

MAPK Signaling Cascades01:07

MAPK Signaling Cascades

Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
8.3K
Notch Signaling Pathway03:14

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
6.6K
Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
10.1K
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
7.4K
Skin Cancer01:30

Skin Cancer

Skin cancer is a type of cancer that occurs when there is an abnormal growth of skin cells, usually triggered by damage to the DNA within the skin cells. It is primarily caused by exposure to ultraviolet (UV) radiation from the sun or artificial sources like tanning beds. Skin cancer is the most common type of cancer worldwide, and its incidence continues to rise.
Basal Cell Carcinoma (BCC): BCC is the most common type of skin cancer, accounting for about 80% of cases. It typically develops in...
5.9K
Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
8.5K