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Particle Agglutination Method for Poliovirus Identification
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Particle Agglutination Method for Poliovirus Identification

Published on: April 20, 2011

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Continued use of poliovirus after eradication: hyper-attenuated strains as a safe alternative for release testing of

Maria R Farcet1, Jens Modrof1, Philip O Rabel1

  • 1Global Pathogen Safety, Vienna, Austria.

Transfusion
|December 12, 2018
PubMed
Abstract

Insights

Hyper-attenuated poliovirus strains (S19) can replace wild-type poliovirus for immunoglobulin lot release testing. This ensures continued safe use of poliovirus while supporting global eradication efforts.

Area of Science:

  • Virology
  • Immunology

Background:

  • Wild-type poliovirus eradication is imminent, necessitating strict containment or discontinuation of laboratory use.
  • US regulations require replicating poliovirus for immunoglobulin lot release testing.
  • Investigating S19 poliovirus strains as alternatives to wild-type virus is crucial.

Purpose of the Study:

  • To assess the suitability of S19 hyper-attenuated poliovirus strains for immunoglobulin lot release assays.
  • To determine if S19 strains can serve as safe and effective replacements for wild-type poliovirus.

Main Methods:

  • S19 poliovirus strains were propagated under good virological practices.
  • Massively parallel sequencing confirmed the S19 hyper-attenuated genotype.
  • The attenuated phenotype was validated in a sensitive mouse model.

Main Results:

  • Propagation and characterization of S19 poliovirus stocks were successful.
  • S19 strains demonstrated an attenuated phenotype in vivo.
  • S19 and wild-type polioviruses showed equivalent performance in lot release assays.

Conclusions:

  • Hyper-attenuated S19 poliovirus stocks are suitable alternatives for immunoglobulin lot release testing.
  • These characterized S19 stocks reconcile containment measures with essential poliovirus use.
  • Deployment of S19 strains supports both poliovirus eradication and ongoing laboratory needs.

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