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Long noncoding RNA ATB participates in the development of renal cell carcinoma by downregulating p53 via binding to

Chao Song1, Yunhe Xiong1, Wenbiao Liao1

  • 1Department of Urology, Renmin Hospital of Wuhan University, Wuhan, People's Republic of China.

Journal of Cellular Physiology
|December 12, 2018
PubMed

Insights

Long noncoding RNA ATB promotes renal cell carcinoma (RCC) progression by enhancing cell proliferation and migration while inhibiting apoptosis. It achieves this by binding to DNMT1, which then downregulates p53 expression.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long noncoding RNAs (lncRNAs) are implicated in various disease pathologies.
  • Aberrant expression of lncRNA ATB has been observed in renal cell carcinoma (RCC).

Purpose of the Study:

  • To investigate the role of lncRNA ATB in regulating the proliferative and migratory capacities of RCC cells.
  • To elucidate the molecular mechanism by which lncRNA ATB influences RCC cell cycle and apoptosis.
  • To examine the interaction between lncRNA ATB, DNMT1, and p53 in RCC.

Main Methods:

  • Cell Counting Kit-8 (CCK-8), 5-ethynyl-2'-deoxyuridine (EdU), and migration assays were used to assess cellular functions.
  • Flow cytometry was employed to analyze cell cycle and apoptosis.
  • RNA immunoprecipitation (RIP), chromatin immunoprecipitation (ChIP), and western blot analyses were performed to study molecular interactions.

Main Results:

  • lncRNA ATB knockdown inhibited proliferation and migration, and promoted apoptosis in RCC cells.
  • lncRNA ATB overexpression enhanced proliferation and migration, and inhibited apoptosis in RCC cells.
  • lncRNA ATB binds to DNMT1, stabilizing its expression and promoting DNMT1-p53 binding, ultimately downregulating p53.

Conclusions:

  • High expression of lncRNA ATB accelerates proliferation and migration while inhibiting apoptosis in RCC cells.
  • lncRNA ATB exerts its oncogenic effects by downregulating p53 through interaction with DNMT1.
  • Targeting lncRNA ATB may represent a potential therapeutic strategy for renal cell carcinoma.
Keywords:
RCCapoptosislncRNA ATBp53proliferation

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