Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Inflammation01:38

Inflammation

62.1K
Overview
62.1K
Complement System01:27

Complement System

10.6K
The complement system is a group of approximately 20 plasma proteins that strengthen the body's defenses against infections through opsonization, inflammation, and cell lysis. Opsonization involves coating pathogens with complement proteins, making them more recognizable and facilitating phagocyte engulfment. Certain complement proteins induce inflammation that attracts immune cells to the site of infection. Cell lysis involves the destruction of pathogens through the formation of a...
10.6K
Complementation Tests00:49

Complementation Tests

6.2K
A complementation test is a simple cross to identify whether the two mutations are located on the same gene or different genes. It was first performed by Edward Lewis in the 1940s while working on fruit flies. He developed the test to identify the location and arrangement of different mutations on chromosomes.
Organisms heterozygous for different mutations are crossed pairwise in all combinations. If present on different genes, the mutations can complement each other by providing the missing...
6.2K
Transcription Factors02:16

Transcription Factors

82.7K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
82.7K
Factors Affecting Protein-Drug Binding: Drug Interactions01:23

Factors Affecting Protein-Drug Binding: Drug Interactions

602
Drug interactions are a critical aspect of pharmacology and can occur when two or more drugs compete for the same binding site. This competition can result in one drug displacing another, altering the effect of the displaced drug. Drug interactions are complex processes that rely heavily on how much of the displacer drug is present and how strongly it can bind to the same sites as the displaced drug.
Displacement interactions can have varying outcomes, ranging from toxicity to virtually...
602
Factors Affecting Renal Clearance: Drug Distribution and Drug Interactions01:09

Factors Affecting Renal Clearance: Drug Distribution and Drug Interactions

533
Renal clearance plays a pivotal role in drug elimination from the body and can be influenced by drug distribution and interactions. Understanding these factors is crucial in pharmacology as they impact the effectiveness and duration of drug therapy.
One important factor is the relationship between renal clearance and the apparent volume of distribution. Renal clearance tends to be inversely proportional to the apparent volume of distribution. Drugs with an extensive distribution volume or those...
533

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Precision Immunoregulation in transplantation: The rise of engineered Treg therapies.

Molecular biology reports·2026
Same author

Mitigation of Ischemia-Reperfusion Injury and Improvement in Overall Graft Viability by Hypothermic Pulsatile Perfusion with Molecular Hydrogen Is Associated with Trx-1/HO-1 Activation in a Non-Survival Ex Vivo Swine Model of Donation-After-Circulatory-Death Kidney Preservation and Transplantation.

International journal of molecular sciences·2026
Same author

TSG-6 promotes Fascin-1-Mediated Microvascular Repair in Murine Airway Allografts.

American journal of respiratory cell and molecular biology·2026
Same author

Multivariate generalized linear model-based discrimination of striped dwarf catfish Mystus vittatus stocks from the River Ganga using body morphometrics.

Journal of fish biology·2026
Same author

CD28 pathway blockade to transplant tolerance: Expanding the landscape of immune regulation.

Current research in translational medicine·2026
Same author

Cellular and Molecular Changes Induced by Various Preservation Temperatures and Methods of Preservation in Renal Grafts and Other Solid Organ Grafts.

International journal of molecular sciences·2026

Related Experiment Video

Updated: Feb 1, 2026

Mouse Model of Alloimmune-induced Vascular Rejection and Transplant Arteriosclerosis
07:05

Mouse Model of Alloimmune-induced Vascular Rejection and Transplant Arteriosclerosis

Published on: May 17, 2015

8.9K

Complement factor and T-cell interactions during alloimmune inflammation in transplantation.

Mohammad Afzal Khan1, Talal Shamma1

  • 1Organ Transplant Research Section, King Faisal Specialist Hospital and Research Centre, Riyadh, Kingdom of Saudi Arabia.

Journal of Leukocyte Biology
|December 12, 2018
PubMed
Summary

Complement factors and T-cell signaling are crucial in transplant rejection. Targeting these interactions may offer a novel therapy to prevent chronic rejection and protect organs.

Keywords:
T-cell activationcomplement mediatorscostimulatory signals

More Related Videos

A Microphysiological System to Study Leukocyte-Endothelial Cell Interaction during Inflammation
12:55

A Microphysiological System to Study Leukocyte-Endothelial Cell Interaction during Inflammation

Published on: December 9, 2021

3.8K
Bimolecular Fluorescence Complementation BiFC Assay for Protein-Protein Interaction in Onion Cells Using the Helios Gene Gun
10:39

Bimolecular Fluorescence Complementation BiFC Assay for Protein-Protein Interaction in Onion Cells Using the Helios Gene Gun

Published on: June 12, 2010

20.2K

Related Experiment Videos

Last Updated: Feb 1, 2026

Mouse Model of Alloimmune-induced Vascular Rejection and Transplant Arteriosclerosis
07:05

Mouse Model of Alloimmune-induced Vascular Rejection and Transplant Arteriosclerosis

Published on: May 17, 2015

8.9K
A Microphysiological System to Study Leukocyte-Endothelial Cell Interaction during Inflammation
12:55

A Microphysiological System to Study Leukocyte-Endothelial Cell Interaction during Inflammation

Published on: December 9, 2021

3.8K
Bimolecular Fluorescence Complementation BiFC Assay for Protein-Protein Interaction in Onion Cells Using the Helios Gene Gun
10:39

Bimolecular Fluorescence Complementation BiFC Assay for Protein-Protein Interaction in Onion Cells Using the Helios Gene Gun

Published on: June 12, 2010

20.2K

Area of Science:

  • Immunology
  • Transplantation Biology
  • Molecular Medicine

Background:

  • Alloimmune responses post-transplantation involve complement factors and T-cell signaling, contributing to transplant-associated injury and chronic rejection (CR).
  • Activated complement factors (C3a, C5a) bind to receptors (C3aR, C5aR) on lymphocytes, including T-regulatory cells (Tregs), influencing immune responses.
  • These interactions modulate Th1-effector cell and Treg activities, leading to inflammation, microvascular damage, and fibrosis.

Purpose of the Study:

  • To review the critical role of complement-T cell interactions in the alloimmune response during transplantation.
  • To explore how these interactions influence effector immune responses and contribute to CR.
  • To identify complement-mediated cell signaling as a potential therapeutic target for preventing CR.

Main Methods:

  • Literature review focusing on complement-T cell interactions in transplantation.
  • Analysis of molecular mechanisms involving complement factors (C3a, C5a) and their receptors (C3aR, C5aR) on T-cells.
  • Examination of the impact on immune cell activity (Th1, Tregs) and downstream inflammatory processes.

Main Results:

  • Complement factors significantly modulate T-cell responses during alloimmune inflammation.
  • The interaction between complement and T-cells drives inflammation, microvascular impairment, and fibrotic remodeling.
  • Dysregulation of complement-T cell signaling is a key factor in the progression of CR.

Conclusions:

  • Complement-T cell interactions are pivotal in determining the outcome of alloimmune responses in transplantation.
  • Targeting complement-mediated cell signaling presents a promising therapeutic strategy to mitigate inflammation and prevent CR.
  • Modulating these interactions could protect transplanted organs and improve long-term graft survival.