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Related Experiment Videos

Biotyping coagulase-negative staphylococci.

G A Hébert1, R C Cooksey, N C Clark

  • 1Hospital Infections Program, Centers for Disease Control, Atlanta, Georgia 30333.

Journal of Clinical Microbiology
|October 1, 1988
PubMed
Summary
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This study defines coagulase-negative staphylococci biotypes using biochemical profiles, adherence, and hemolysis tests. Biotyping helps assess the clinical significance of these common bacteria, aiding in infection control.

Area of Science:

  • Clinical Microbiology
  • Bacteriology
  • Molecular Typing

Background:

  • Coagulase-negative staphylococci (CoNS) are significant opportunistic pathogens.
  • Accurate biotyping is crucial for understanding CoNS pathogenicity and clinical relevance.
  • Current methods may not fully differentiate CoNS species and strains.

Purpose of the Study:

  • To establish a biotyping scheme for 1,064 clinical isolates of eight CoNS species.
  • To evaluate the utility of biochemical profiles, adherence, and synergistic hemolysis tests for CoNS biotyping.
  • To correlate biotyping results with antimicrobial susceptibility and plasmid profiles for Staphylococcus epidermidis.

Main Methods:

  • Utilized Staph-Ident panels for biochemical profiling.
  • Incorporated adherence and synergistic hemolysis assays.

Related Experiment Videos

  • Analyzed antimicrobial susceptibility and plasmid profiles for selected S. epidermidis clusters.

    • Main Results:

    • Defined 69 biotypes among 672 Staphylococcus epidermidis isolates.
    • Other CoNS species exhibited fewer biotypes due to more uniform test results.
    • Biotyping, antimicrobial, and plasmid profiles showed varying discriminatory power across S. epidermidis clusters.

    Conclusions:

    • Biochemical profiles combined with adherence and hemolysis tests provide a robust biotyping scheme for CoNS.
    • Adherence and synergistic hemolysis may serve as indices for clinical significance.
    • Integrated typing methods (biochemical, antimicrobial, plasmid) offer complementary insights into CoNS epidemiology and pathogenicity.