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Platelet-derived microparticles generated in vitro resemble circulating vesicles of patients with rheumatoid

Juan Villar-Vesga1, Camilo Grajales1, Catalina Burbano2

  • 1Grupo de Inmunología Celular e Inmunogenética, Instituto de Investigaciones Médicas, Facultad de Medicina, Universidad de Antioquia UdeA, Calle 70 No 52-21, Medellín, Colombia.

Cellular Immunology
|December 13, 2018
PubMed

Insights

Rheumatoid arthritis patients exhibit elevated immune complexes involving platelet-derived microparticles (PMPs) and citrullinated peptides (CPs). These PMPs-ICs activate monocytes differently in patients versus healthy individuals, suggesting distinct immune responses.

Area of Science:

  • Immunology
  • Rheumatology
  • Cell Biology

Background:

  • Rheumatoid arthritis (RA) is characterized by immune system dysregulation.
  • Platelet-derived microparticles (PMPs) carrying citrullinated peptides (CPs) form immune complexes (PMPs-ICs) and are elevated in RA patients.
  • Monocytes are key inflammatory mediators involved in clearing PMPs-ICs.

Purpose of the Study:

  • To generate PMPs-ICs in vitro.
  • To investigate the effects of PMPs-ICs on monocytes from RA patients and healthy individuals (HI).

Main Methods:

  • Characterization of PMPs from RA patients and HI.
  • In vitro generation of PMPs-ICs.
  • Analysis of monocyte responses (cytokine production, gene/protein expression) to PMPs-ICs in RA patients and HI.

Main Results:

  • PMPs from RA patients and HI showed similar basic characteristics.
  • RA patient PMPs had a higher frequency of IgG and CPs compared to HI.
  • In vitro generated PMPs-ICs mirrored patient circulating PMPs in IgG and CP positivity.
  • PMPs-ICs induced pro-inflammatory cytokines and CX3CR1 in HI monocytes.
  • PMPs-ICs upregulated IL-10 and CD36 in RA patient monocytes.

Conclusions:

  • PMPs-ICs activate monocytes in both healthy individuals and RA patients.
  • Monocyte activation by PMPs-ICs elicits a pro-inflammatory response in healthy individuals.
  • Monocyte response to PMPs-ICs in RA patients is characterized by IL-10 and CD36 upregulation, suggesting a more tolerant or regulatory phenotype.
Keywords:
Citrullinated peptidesImmune complexesMicroparticlesMonocytesPlatelet-derived microparticlesRheumatoid arthritis

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