Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Co-activators and Co-repressors02:04

Co-activators and Co-repressors

8.6K
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
8.6K
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

3.0K
3.0K
Prokaryotic Transcriptional Activators and Repressors01:58

Prokaryotic Transcriptional Activators and Repressors

25.5K
The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
25.5K
Prokaryotic Transcriptional Activators and Repressors01:58

Prokaryotic Transcriptional Activators and Repressors

10.6K
10.6K
Constitutive and Regulated Gene Expression01:27

Constitutive and Regulated Gene Expression

1.2K
Gene expression in prokaryotes is governed by constitutive and regulated systems, allowing cells to balance the production of essential proteins with adaptive responses to environmental changes.Constitutive Gene ExpressionConstitutive, or housekeeping, genes are continuously expressed as they encode proteins vital for fundamental cellular processes. These include enzymes for glycolysis, ribosomal components for protein synthesis, and proteins involved in DNA replication. Their constant...
1.2K
Combinatorial Gene Control02:33

Combinatorial Gene Control

9.7K
Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
9.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cross-Modality Treatment Generalization in Sentence Comprehension in Aphasia: Evidence From Structural Priming and Webcam-Based Eye-Tracking.

International journal of language & communication disorders·2026
Same author

Peritoneal strumosis and the role of imaging in the diagnostic workup: A case report.

Radiology case reports·2026
Same author

Evaluating the utility of large language models for detecting and simulating language dysfunction.

Frontiers in artificial intelligence·2026
Same author

Engineering Cofacial Trizinc(II)porphyrin Trimers: Linking Molecular Architecture with Excited-State Dynamics.

Inorganic chemistry·2026
Same author

First aid educational needs among nursing officers in military primary clinics: a mixed-methods study.

BMC nursing·2026
Same author

Leonurine-Standardized <i>Leonurus japonicus</i> Extract Promotes Recovery from Immobilization-Induced Muscle Atrophy via PI3K/Akt/mTOR Signaling in Mice.

Journal of microbiology and biotechnology·2026

Related Experiment Video

Updated: Feb 1, 2026

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
08:54

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

Published on: March 29, 2019

7.6K

Procaspase activating compound 1 controls tetracycline repressor-regulated gene expression system.

Chiman Song1,2, Namkyoung Kim3, Miri Park1

  • 1Chemical Kinomics Research Center, Korea Institute of Science and Technology, 5 Hwarangro 14-gil, Seongbuk-gu, Seoul 02792, Republic of Korea.

Bioscience Reports
|December 13, 2018
PubMed
Summary

Procaspase activating compound 1 (PAC-1) is a novel small molecule that rapidly reduces TetR-regulated gene expression in mammalian cells. This discovery offers precise control over gene expression by efficiently turning off target genes, overcoming limitations of existing methods.

Keywords:
PAC-1TetR-regulated systemantagonistanti-inductiongene regulationtetracycline repressor

More Related Videos

Generation of Stable Human Cell Lines with Tetracycline-inducible Tet-on shRNA or cDNA Expression
09:51

Generation of Stable Human Cell Lines with Tetracycline-inducible Tet-on shRNA or cDNA Expression

Published on: March 5, 2013

36.1K
Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells
10:34

Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells

Published on: April 14, 2010

16.0K

Related Experiment Videos

Last Updated: Feb 1, 2026

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
08:54

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

Published on: March 29, 2019

7.6K
Generation of Stable Human Cell Lines with Tetracycline-inducible Tet-on shRNA or cDNA Expression
09:51

Generation of Stable Human Cell Lines with Tetracycline-inducible Tet-on shRNA or cDNA Expression

Published on: March 5, 2013

36.1K
Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells
10:34

Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells

Published on: April 14, 2010

16.0K

Area of Science:

  • Molecular Biology
  • Gene Regulation
  • Cell Biology

Background:

  • The tetracycline repressor (TetR)-regulated system is crucial for controlling gene expression in research.
  • Existing TetR inducers like tetracycline (Tc) and doxycycline (Dox) have long half-lives, hindering rapid reversal of gene expression.
  • A need exists for small molecules that can efficiently and rapidly shut down TetR-regulated gene expression.

Purpose of the Study:

  • To identify a small molecule capable of rapidly reversing TetR-regulated gene expression in mammalian cells.
  • To demonstrate the efficacy of this molecule in controlling the expression of reporter genes and functional proteins.
  • To establish a new method for precise gene expression regulation.

Main Methods:

  • Utilized TetR-regulated green fluorescent protein (GFP) reporter system in mammalian cells.
  • Investigated the effect of procaspase activating compound 1 (PAC-1) on GFP expression.
  • Applied PAC-1 to regulate the expression of transient receptor potential melastatin 7 (TRPM7) protein.
  • Monitored cellular changes in morphology as an indicator of TRPM7 protein activity.

Main Results:

  • PAC-1 rapidly reduced transient TetR-regulated GFP expression in mammalian cells.
  • PAC-1 effectively decreased TRPM7 protein expression.
  • Observed significant changes in cell morphology correlated with reduced TRPM7 expression.
  • Demonstrated PAC-1 as the first small molecule for efficient "off" control of TetR-regulated gene expression.

Conclusions:

  • PAC-1 is a potent small molecule for rapidly shutting down TetR-regulated gene expression in mammalian systems.
  • This finding provides a valuable tool for precise temporal control of gene expression.
  • PAC-1 enables dynamic studies of gene function by allowing rapid gene silencing.