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Updated: Feb 1, 2026

In Vivo Tracking of Edema Development and Microvascular Pathology in a Model of Experimental Cerebral Malaria Using Magnetic Resonance Imaging
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Evaluation of T cells infiltration inhibition in brain by immunohistochemistry during experimental cerebral malaria

Ruchika Saroa1, Upma Bagai1

  • 1Parasitology Laboratory, Department of Zoology, Panjab University, Chandigarh, 160014 India.

Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology
|December 13, 2018
PubMed

Insights

Experimental cerebral malaria in mice involves neuroinflammation and T cell infiltration in the brain. Artesunate-sulphadoxine-pyrimethamine treatment prevented mortality and brain injury in infected mice.

Area of Science:

  • Neuroscience
  • Immunology
  • Parasitology

Background:

  • Cerebral malaria, a severe complication of Plasmodium infection, has an unknown pathogenesis despite extensive research.
  • Plasmodium berghei ANKA in mice serves as a model for studying cerebral malaria and its neurological effects.

Purpose of the Study:

  • To investigate the pathogenesis of experimental cerebral malaria using histopathological and immunofluorescent staining.
  • To evaluate the effects of artesunate-sulphadoxine-pyrimethamine (AS+SP) treatment on cerebral malaria progression and outcomes.

Main Methods:

  • Histopathological analysis of brain tissue from infected and treated mice.
  • Immunofluorescent staining to identify and localize T cells (CD3+, CD4+, CD8+) in the brain.
  • Monitoring survival rates and clinical signs in infected and treated mouse models.

Main Results:

  • Cerebral vessels in infected mice showed congestion with red blood cells and leukocytes.
  • Increased expression of CD3+, CD4+, and CD8+ T cells was observed in the brain cortex of infected mice compared to treated mice.
  • Artesunate-sulphadoxine-pyrimethamine treatment prevented mortality and histopathological signs of brain injury, with all treated mice surviving for one month.

Conclusions:

  • Neuroinflammation and T cell infiltration are key features of experimental cerebral malaria pathogenesis.
  • Artesunate-sulphadoxine-pyrimethamine is effective in treating experimental cerebral malaria, preventing mortality and brain damage.
  • Understanding T cell localization is crucial for developing targeted therapies for cerebral malaria.
Keywords:
CD3+ FITCDAPIT cells

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