Cloning of myelomas and hybridomas in fibrin clots

Myelomas and hybridomas were observed to proliferate normally when trapped in a fibrin clot. The fibrin clot was obtained by including fibrinogen in the cell culture medium and thrombin in the plastic dish. A clot formed within seconds and cloning efficiency was around 100%. This technique has all the advantages of semi-solid medium cloning but avoids toxicity to the cells and the exposure to high temperature associated with the soft-agar cloning technique.