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The potential protective effect of two actinomycete extracts against carbon tetrachloride-induced hepatotoxicity in

Wael N Hozzein1,2, Areej Abdulkareem Al-Khalaf3, Mohamed Mohany4

  • 1Bioproducts Research Chair, Zoology Department, College of Science, King Saud University, P.O. Box 2455, Riyadh, 11451, Saudi Arabia. hozzein29@yahoo.com.

Environmental Science and Pollution Research International
|December 13, 2018
PubMed

Insights

Two soil actinomycete extracts, S19 and G30, demonstrated significant protective effects against carbon tetrachloride (CCl4)-induced liver damage in male rats by reducing oxidative stress and enhancing antioxidant defenses.

Area of Science:

  • Microbiology
  • Toxicology
  • Pharmacology

Background:

  • Carbon tetrachloride (CCl4) is a known hepatotoxin that induces oxidative stress and liver damage.
  • Soil actinomycetes are a promising source of bioactive compounds with potential therapeutic applications.
  • Investigating natural product-based interventions for liver protection is crucial.

Purpose of the Study:

  • To evaluate the hepatoprotective potential of two actinomycete extracts (S19 and G30) against CCl4-induced liver injury in male rats.
  • To elucidate the underlying mechanisms, including the modulation of oxidative stress markers and antioxidant enzymes.

Main Methods:

  • Sixty-four male rats were divided into four groups: control, CCl4 only, CCl4 + extract S19, and CCl4 + extract G30.
  • Treatment duration was 8 weeks, with biochemical and histopathological assessments performed.
  • Serum enzyme levels (ALT, AST, ALP, LDH), total bilirubin, hepatic antioxidant markers (GSH, GSH-Px, Px, CAT, SOD), and malondialdehyde (MDA) were measured.

Main Results:

  • Both extracts significantly reduced elevated serum liver enzymes (ALT, AST, ALP) induced by CCl4.
  • Extract G30 also significantly decreased serum LDH and total bilirubin levels.
  • Hepatic levels of antioxidant enzymes (GSH, GSH-Px, Px, CAT, SOD) increased, while MDA levels decreased in treated groups.
  • Histopathological examination showed partial reversal of liver lesions, including necrosis and inflammation.

Conclusions:

  • Actinomycete extracts S19 and G30 exhibit significant hepatoprotective effects against CCl4-induced liver damage in rats.
  • The protective mechanism involves the mitigation of oxidative stress and enhancement of the endogenous antioxidant defense system.
  • These findings highlight the therapeutic potential of soil actinomycete-derived compounds for managing liver toxicity.
Keywords:
Actinomycete extractsCCl4HepatotoxicityLiver enzymesOxidative stress

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