Related Experiment Video
Updated: Feb 1, 2026

Generation and Expansion of Primary, Malignant Pleural Mesothelioma Tumor Lines
Published on: April 21, 2022
Processing of Primary Patient Tumors and Subsequent Generation of Primary Cell Lines
Laura Gosa1,2, Lisa Ta1, David A Nathanson3,4
1Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.
Abstract:
Patient tumor tissue processing is an important step in the generation of clinically relevant specimens for in vitro and in vivo studies. Proper disassociation and tissue sample cleanup is a multistep, time-consuming process that ultimately effects the generation of patient derived xenografts and neurosphere cultures. Here we describe a detailed protocol on how to process and disassociate patient glioma tissue and subsequent steps on orthotopic implantation and in vitro generation of neurospheres.
Related Concept Videos
Primary Active Transport
Primary Active Transport
Primary and Secondary Growth in Roots and Shoots
Primary Production
Primary Distribution
Nomenclature of Primary Amines

