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Isolation of Antigen-Specific, Antibody-Secreting Cells Using a Chip-Based Immunospot Array

Hiroyuki Kishi1, Tatsuhiko Ozawa2, Hiroshi Hamana2

  • 1Department of Immunology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan. immkishi@med.u-toyama.ac.jp.

Methods in Molecular Biology (Clifton, N.J.)
|December 13, 2018
PubMed

Antigen-specific monoclonal antibodies are useful tools to detect very small amounts of antigenic materials and are applicable for antibody therapeutics. To produce mouse monoclonal antibodies, a hybridoma between B lymphocytes and myeloma cells is used to produce antigen-specific monoclonal antibodies. However, a good hybridoma system is not available to obtain human monoclonal antibodies. To produce antigen-specific human monoclonal antibodies, transformation of B lymphocytes with Epstein-Barr viruses or a phage-display system is used. Here, we describe the screening of antigen-specific, antibody-secreting cells using microwell array chips to obtain antigen-specific human monoclonal antibodies. The system can be applied to screen antigen-specific, antibody-secreting cells from any animal species.

Keywords:
Antibody-secreting cellAntigen-specific antibodyImmunospot array assay on a chipMicrowell-array chip

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