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Isolation of Antigen-Specific, Antibody-Secreting Cells Using a Chip-Based Immunospot Array
Hiroyuki Kishi1, Tatsuhiko Ozawa2, Hiroshi Hamana2
1Department of Immunology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan. immkishi@med.u-toyama.ac.jp.
Methods in Molecular Biology (Clifton, N.J.)
|December 13, 2018
Summary
Researchers developed a novel microwell array chip system for efficiently screening antigen-specific antibody-secreting cells. This breakthrough facilitates the production of human monoclonal antibodies for therapeutic applications.
Area of Science:
- Immunology
- Biotechnology
- Cell Biology
Background:
- Monoclonal antibodies are crucial for detecting antigens and developing antibody therapeutics.
- Current methods for producing human monoclonal antibodies, such as Epstein-Barr virus transformation or phage display, have limitations.
- A robust system for generating antigen-specific human monoclonal antibodies is needed.
Purpose of the Study:
- To develop and describe a novel screening system for identifying antigen-specific, antibody-secreting cells.
- To enable the efficient production of antigen-specific human monoclonal antibodies.
- To provide a versatile platform applicable to various animal species.
Main Methods:
- Utilized microwell array chips for high-throughput screening of antibody-secreting cells.
- Focused on isolating B lymphocytes producing specific antibodies against target antigens.
- Developed a system for screening antigen-specific B cells from various species.
Main Results:
- Successfully demonstrated the screening of antigen-specific, antibody-secreting cells using microwell array chips.
- Established a method for obtaining antigen-specific human monoclonal antibodies.
- The developed system is adaptable for screening cells from different animal species.
Conclusions:
- Microwell array chip technology offers an effective approach for screening antigen-specific antibody-secreting cells.
- This system significantly advances the production of human monoclonal antibodies.
- The platform's species-agnostic nature broadens its applicability in immunological research and therapeutic development.
Keywords:
Antibody-secreting cellAntigen-specific antibodyImmunospot array assay on a chipMicrowell-array chipMore Related Videos
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