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Published on: February 5, 2014
Analysis of Anti-C1q Autoantibodies by Western Blot
Anci Verlemyr1, Lennart Truedsson2, Lillemor Skattum3,4
1Clinical Immunology and Transfusion Medicine, Region Skåne, Sweden.
Abstract:
Anti-C1q autoantibodies may be found in many conditions, most commonly in systemic lupus erythematosus (SLE) and hypocomplementemic urticarial vasculitis syndrome (HUVS), and are diagnostic markers as well as disease activity markers in lupus nephritis. Sera from patients with SLE and HUVS show partly distinct autoantibody reactivities to separated protein chains B and C of the first component of complement, C1q. These different binding specificities can be detected by Western blot analysis of the autoantibodies under reducing conditions. Results may help clinicians to differentiate between SLE and HUVS.
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