Pedobacter miscanthi sp. nov., isolated from Miscanthus sinensis
Zhaojin Chen1, Wei Tian2, Feng Sun1
1Innovation Center of Water Security for Water Source Region of Mid-route Project of South-North Water Diversion of Henan Province, Key Laboratory of Ecological Security for Water Source Region of Mid-line of South-to-North Diversion Project of Henan Province, School of Agricultural Engineering, Nanyang Normal University, Nanyang 473061, PR China.
International Journal of Systematic and Evolutionary Microbiology
|December 13, 2018
Summary
A novel bacterium, RS10, was isolated from Miscanthus sinensis. Phylogenetic and genomic analyses confirmed it represents a new species, Pedobacter miscanthi, expanding the Pedobacter genus.
Area of Science:
- Microbiology
- Plant-associated bacteria
- Bacterial taxonomy
Background:
- Endophytic bacteria reside within plant tissues, playing diverse roles in plant health and microbial ecology.
- The genus Pedobacter comprises Gram-negative bacteria known for their ecological significance.
Purpose of the Study:
- To isolate and characterize novel endophytic bacteria from Miscanthus sinensis.
- To determine the taxonomic placement of a newly isolated bacterial strain through polyphasic characterization.
Main Methods:
- Isolation and cultivation of endophytic bacteria from Miscanthus sinensis.
- Phylogenetic analysis using 16S rRNA gene sequencing.
- Fatty acid analysis, isoprenoid quinone profiling, polar lipid analysis, and DNA G+C content determination.
Main Results:
- A novel Gram-negative, aerobic bacterium, strain RS10, was isolated.
- Phylogenetic analysis indicated strain RS10 belongs to the genus Pedobacter, with high similarity to known species.
- Distinct phenotypic characteristics, including major fatty acids (iso-C15:0, iso-C17:0-3OH, and summed feature 3) and MK-7 as the sole isoprenoid quinone, supported its novelty.
Conclusions:
- Strain RS10 represents a new species within the genus Pedobacter.
- The proposed name for this novel species is Pedobacter miscanthi sp. nov., with RS10 as the type strain.
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