Antibiotic resistance of Campylobacter jejuni isolates recovered from humans with diarrhoea in Turkey

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Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics MSPP
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Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics MSPP

Published on: October 30, 2016

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Tuba Kayman1, Seçil Abay2, Fuat Aydin2

  • 11​University of Health Sciences, Şişli Hamidiye Etfal Training and Research Hospital, Medical Microbiology Clinic, Istanbul, Turkey.

Journal of Medical Microbiology
|December 13, 2018
PubMed
Abstract

Insights

High resistance to ciprofloxacin and tetracycline was observed in Campylobacter jejuni clinical isolates from Turkey. Genetic analysis confirmed specific mutations and genes responsible for antibiotic resistance, suggesting erythromycin as a potential first-line treatment.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Molecular Biology

Background:

  • Campylobacter jejuni is a leading cause of bacterial gastroenteritis worldwide.
  • Antibiotic resistance in C. jejuni poses a significant public health threat.
  • Understanding resistance mechanisms is crucial for effective treatment strategies.

Purpose of the Study:

  • To investigate the prevalence and genetic basis of resistance to ciprofloxacin, tetracycline, and erythromycin in clinical C. jejuni isolates from Turkey.
  • To correlate phenotypic resistance with specific genetic mutations and genes.

Main Methods:

  • Phenotypic susceptibility testing using E-test to determine Minimum Inhibitory Concentrations (MICs).
  • Polymerase Chain Reaction (PCR) screening for resistance genes and mutations.
  • DNA sequencing for detailed analysis of identified genetic markers.

Main Results:

  • High rates of resistance were found: 74.3% to ciprofloxacin, 25% to tetracycline, and 5.9% to erythromycin.
  • Ciprofloxacin resistance was associated with the Thr-86-Ile mutation in the gyrA gene.
  • Tetracycline resistance was linked to the presence of the tetO gene.
  • Erythromycin resistance was attributed to the A2075G mutation in the 23S rRNA gene.

Conclusions:

  • Phenotypic and genetic resistance detection methods showed strong agreement.
  • A high prevalence of ciprofloxacin and moderate tetracycline resistance was observed in Turkish C. jejuni isolates.
  • Low-level erythromycin resistance suggests its potential utility as a primary treatment option for C. jejuni infections in the region.
Keywords:
Campylobacter jejuniE-testMAMA-PCRerythromycingyrAtetracycline

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