Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Oligomerization of oncoprotein p53.

S Kraiss¹, A Quaiser, M Oren

¹Department of Biochemistry, University of Ulm, Federal Republic of Germany.

Journal of Virology

|December 1, 1988

Summary

Cellular phosphoprotein p53 exists in different molecular forms. Transformed cells show high-molecular-weight p53, suggesting a link between p53 oligomerization and cell transformation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Usefulness of the 'two-step method' of digital follow-up for early-stage melanoma detection in high-risk French patients: a retrospective 4-year study.

The British journal of dermatology·2019

Same author

[What is established in cell therapies? : Possibilities and limits in immuno-oncology].

Der Internist·2018

Same author

let-7b and let-7c microRNAs promote histone H2B ubiquitylation and inhibit cell migration by targeting multiple components of the H2B deubiquitylation machinery.

Oncogene·2017

Same author

On the generalizability of prostate cancer studies: why race matters.

Annals of oncology : official journal of the European Society for Medical Oncology·2016

Same author

Percentiles of Lymphocyte Subsets in Preterm Infants According to Gestational Age Compared to Children and Adolescents.

Scandinavian journal of immunology·2016

Same author

Effect of teleradiology upon pattern of transfer of head injured patients from a rural general hospital to a neurosurgical referral centre: follow-up study.

Emergency medicine journal : EMJ·2015

Area of Science:

Molecular Biology
Cell Biology
Protein Chemistry

Background:

The tumor suppressor protein p53 is multifunctional and can exist in various structural forms.
Overexpression of p53 in immortalized and transformed cell lines enables detailed structural analysis.

Purpose of the Study:

To investigate the quaternary structure of p53 in different cell types.
To determine the relationship between p53 complex formation and the transformation state of cells.

Main Methods:

Sucrose density gradient centrifugation to analyze protein complexes.
Monoclonal antibodies to differentiate p53 forms (free vs. complexed).
Pulse-labeling experiments to study protein oligomerization kinetics.

Main Results:

Simian virus 40-transformed cells exhibit both high- and low-molecular-weight p53 forms.
p53 oligomerization is rapid, with monomers quickly forming stable high-molecular-weight structures.
p53 is predominantly in high-molecular-weight forms in transformed cells, but low-molecular-weight forms in normal and immortalized cells.
Complex formation requires post-translational modification of p53 or T antigen.

Conclusions:

The cellular concentration of p53 influences T-p53 complex levels.
High-molecular-weight p53 forms correlate with the transformed state of a cell.
p53 oligomerization is a rapid process essential for its function in cellular transformation.

Related Experiment Videos