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Updated: Feb 1, 2026

A Quantitative Measurement of Reactive Oxygen Species and Senescence-associated Secretory Phenotype in Normal Human Fibroblasts During Oncogene-induced Senescence
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TRIB2 functions as novel oncogene in colorectal cancer by blocking cellular senescence through AP4/p21 signaling

Zhenlin Hou1, Kaixuan Guo1, Xuling Sun1

  • 1Cancer Research Institute, Tongji Hospital, Huazhong University of Science and Technology, Wuhan, China.

Molecular Cancer
|December 14, 2018
PubMed
Abstract

Insights

Tribbles homolog 2 (TRIB2) suppresses cellular senescence in colorectal cancer (CRC) by inhibiting p21 expression via AP4 interaction. Targeting TRIB2 may offer a new therapeutic strategy for CRC patients.

Area of Science:

  • Oncology
  • Cell Biology
  • Molecular Mechanisms

Background:

  • Cellular senescence is a key mechanism for tumor suppression.
  • Tribbles homolog 2 (TRIB2) influences cancer cell proliferation and drug resistance.
  • The role of TRIB2 in colorectal cancer (CRC) senescence and its underlying mechanisms are not well understood.

Purpose of the Study:

  • To investigate the role of TRIB2 in cellular senescence of colorectal cancer.
  • To elucidate the molecular mechanism by which TRIB2 regulates senescence in CRC.
  • To evaluate TRIB2 as a potential therapeutic target for CRC.

Main Methods:

  • Immunohistochemistry and RT-PCR to assess TRIB2 expression in CRC tissues.
  • Cell Counting Kit-8, flow cytometry, and senescence-associated β-galactosidase staining to evaluate cellular senescence.
  • Western blot, RT-PCR, luciferase assay, immunoprecipitation, and chromatin-immunoprecipitation to determine TRIB2's regulatory mechanism on p21 and AP4 interaction.

Main Results:

  • TRIB2 expression is upregulated in CRC tissues and correlates with poor prognosis.
  • TRIB2 depletion inhibits CRC cell proliferation and induces senescence, while TRIB2 overexpression promotes proliferation and blocks senescence.
  • TRIB2 interacts with AP4, inhibiting p21 expression and consequently suppressing cellular senescence.

Conclusions:

  • TRIB2 suppresses cellular senescence in CRC by interacting with AP4 to downregulate p21 expression.
  • TRIB2 represents a potential therapeutic target for colorectal cancer treatment.
Keywords:
AP4Cellular senescenceColorectal cancerTRIB2p21p53

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