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Differences in micro-RNA expression profile between vastus lateralis samples and myotubes in COPD cachexia.

Esther Barreiro1,2, Antonio Sancho-Muñoz1, Ester Puig-Vilanova1

  • 1Pulmonology Department, Muscle Wasting and Cachexia in Chronic Respiratory Diseases and Lung Cancer Research Group, Institut Hospital del Mar d'Investigacions Mèdiques-Hospital del Mar, Parc de Salut Mar, Health and Experimental Sciences Department, Universitat Pompeu Fabra, Barcelona Biomedical Research Park, Barcelona , Spain.

Journal of Applied Physiology (Bethesda, Md. : 1985)
|December 14, 2018
PubMed
Summary

Muscle wasting in chronic obstructive pulmonary disease (COPD) is linked to altered micro-RNA expression in vivo. However, these differences in micro-RNA profiles disappear in cell cultures, suggesting environmental factors drive COPD muscle changes.

Keywords:
lower-limb muscle atrophymuscle specimensmuscle-enriched micro-RNAsmyotubessevere COPD

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Pulmonology

Background:

  • Quadriceps muscle weakness and wasting are common in chronic obstructive pulmonary disease (COPD).
  • Micro-RNAs (miRNAs) are implicated in muscle growth and differentiation, potentially influencing muscle mass.
  • Understanding miRNA profiles in COPD muscle is crucial for developing targeted therapies.

Purpose of the Study:

  • To investigate differences in muscle-enriched miRNA expression in vastus lateralis muscle biopsies and cultured myotubes between COPD patients and healthy controls.
  • To determine if in vivo environmental factors influence miRNA expression in COPD-related muscle wasting.
  • To assess the potential of myotube miRNA profiles as biomarkers or therapeutic targets.

Main Methods:

  • Recruited 29 COPD patients (15 with muscle wasting, 14 with normal body composition) and 10 healthy controls.
  • Obtained vastus lateralis muscle biopsies for in vivo analysis and primary cell cultures for in vitro myotube differentiation.
  • Analyzed expression of miR-1, miR-133a, miR-206, miR-486, miR-29b, miR-27a, and miR-181a using quantitative real-time PCR.

Main Results:

  • Upregulation of miR-1, miR-206, miR-486, and miR-29b was observed in muscle biopsies of COPD patients compared to controls.
  • No significant differences in the expression of studied miRNAs were found in myotubes between COPD patients and controls.
  • This suggests in vivo environmental factors, not intrinsic cellular differences, account for altered miRNA expression in COPD muscle.

Conclusions:

  • Environmental factors (e.g., blood flow, metabolism, inflammation) in vivo significantly influence muscle-enriched miRNA expression in COPD.
  • Myotube miRNA profiles are similar between COPD patients and controls, indicating they may not be direct targets for therapeutic intervention in muscle wasting.
  • Therapeutic strategies for COPD muscle wasting should focus on modulating these environmental factors rather than solely targeting miRNA expression.