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Related Concept Videos

CRISPR01:59

CRISPR

57.9K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

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CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
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CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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Symbiosis00:58

Symbiosis

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Symbiotic relationships are long-term, close interactions between individuals of different species that affect the distribution and abundance of those species. When a relationship is beneficial to both species, this is called mutualism. When the relationship is beneficial to one species but neither beneficial nor harmful to the other species, this is called commensalism. When one organism is harmed to benefit another, the relationship is known as parasitism. These types of relationships often...
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What is Genetic Engineering?00:49

What is Genetic Engineering?

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Overview
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Related Experiment Video

Updated: Feb 1, 2026

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
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CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

Published on: December 11, 2020

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CRISPRing the Elephant in the Room.

Oliver Billker1

  • 1Molecular Infection Medicine Sweden (MIMS), Department of Molecular Biology, Umeå University, Umeå, SE-901 87, Sweden.

Cell Host & Microbe
|December 14, 2018
PubMed
Summary

Guanylyl-cyclases (GCs) are crucial in apicomplexa parasites like Toxoplasma and Plasmodium. Genome editing with CRISPR-Cas9 elucidated the roles of these large genes in parasite biology.

Area of Science:

  • Parasitology
  • Molecular Biology
  • Genetics

Background:

  • Guanylyl-cyclases (GCs) are enzymes with vital roles in cellular signaling.
  • The functions of GCs in apicomplexa, such as Toxoplasma and Plasmodium, are poorly understood due to large gene sizes.

Purpose of the Study:

  • To investigate the roles of guanylyl-cyclases (GCs) in apicomplexa.
  • To overcome challenges posed by the large size of GC genes in these parasites.

Main Methods:

  • Utilized CRISPR-Cas9 genome editing technology.
  • Characterized the functions of GCs in Toxoplasma and Plasmodium species.

Main Results:

  • Genome editing enabled the functional characterization of large GC genes.

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Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
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CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
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Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
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  • Specific roles of GCs in apicomplexa were elucidated through these studies.

    • Conclusions:

    • CRISPR-Cas9 is an effective tool for studying large genes in apicomplexa.
    • GCs play significant, previously elusive roles in the biology of Toxoplasma and Plasmodium.