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Colloids03:22

Colloids

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Children at play often make suspensions such as mixtures of mud and water, flour and water, or a suspension of solid pigments in water known as tempera paint. These suspensions are heterogeneous mixtures composed of relatively large particles that are visible to the naked eye or can be seen with a magnifying glass. They are cloudy, and the suspended particles settle out after mixing. On the other hand, a solution is a homogeneous mixture in which no settling occurs and in which the dissolved...
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Children at play often make suspensions such as mixtures of mud and water, flour and water, or a suspension of solid pigments in water known as tempera paint. These suspensions are heterogeneous mixtures composed of relatively large particles visible to the naked eye or seen with a magnifying glass. They are cloudy, and the suspended particles settle out after mixing. The suspended particles in a suspension settle out after some time of mixing. The separation of particles from a suspension is...
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The high insolubility of some precipitates can result in an unfavorable relative supersaturation. This can lead to colloidal particles with a large surface-to-mass ratio, where adsorption is promoted. For instance, in the precipitation of silver chloride, silver ions are adsorbed on the surface of the colloidal particles, forming a primary layer. This layer attracts ions of opposite charge (such as nitrate ions), forming a diffuse secondary layer of adsorbed ions. This electric double layer...
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Ionic Crystal Structures02:42

Ionic Crystal Structures

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Ionic crystals consist of two or more different kinds of ions that usually have different sizes. The packing of these ions into a crystal structure is more complex than the packing of metal atoms that are the same size.
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Inverse Trigonometric Functions01:29

Inverse Trigonometric Functions

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Inverse trigonometric functions are fundamental mathematical tools that reverse the actions of standard trigonometric functions. While trigonometric functions map angles to ratios, inverse trigonometric functions perform the opposite operation by mapping a ratio back to its corresponding angle. These functions are essential in various applications, particularly in determining angles when given specific distances, such as calculating elevation angles in navigation and engineering.For a function...
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Crystal Growth: Principles of Crystallization01:25

Crystal Growth: Principles of Crystallization

5.0K
Crystallization is a phase transformation process in which crystals are precipitated from a supersaturated solution or formed from other sources. During crystallization, atoms or molecules arrange themselves into a well-defined, rigid crystal lattice to minimize energy.
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Fluorescent Aptamer Immobilization on Inverse Colloidal Crystals.

Andrea Chiappini1, Laura Pasquardini2,3, Somayeh Nodehi4

  • 1CNR-IFN CSMFO Lab. and FBK CMM, via alla Cascata 56/C, 38123 Povo Trento, Italy. andrea.chiappini@unitn.it.

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|December 15, 2018
PubMed
Summary

We developed a two-step method to create DNA-aptamer-functionalized silica inverse opals. This approach enables the development of novel fluorescence-based biosensors with tunable optical properties.

Keywords:
DNA-aptamersFDTD simulationsPWE methodband gapco-assemblycolloidal crystalfluorescence

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Area of Science:

  • Materials Science
  • Nanotechnology
  • Biotechnology

Background:

  • Silica inverse opals offer unique photonic properties.
  • DNA aptamers are valuable recognition elements for biosensing.
  • Integrating these materials is challenging.

Purpose of the Study:

  • To develop a versatile two-step method for creating DNA-aptamer-functionalized silica inverse opals.
  • To assess the optical and morphological properties of the functionalized structures.
  • To demonstrate the potential for fluorescence-based biosensing applications.

Main Methods:

  • Co-assembly method for high-quality inverse silica opal fabrication.
  • Epoxy chemistry for functionalization with DNA-aptamers labeled with Cy3 fluorophore.
  • Morphological, optical, photoluminescence, FDTD simulations, and PWE calculations for characterization.

Main Results:

  • Achieved high-quality inverse silica opals with large ordered domains.
  • Confirmed effective immobilization of DNA-aptamers via fluorescence measurements.
  • Demonstrated tunable fluorescence by altering the photonic band gap, linked to density of states (DOS).

Conclusions:

  • The developed two-step approach is a versatile method for functionalizing silica inverse opals.
  • The resulting structures show potential as a platform for fluorescence-based biosensors.
  • This work represents a proof-of-concept for advanced bio-photonic devices.