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Visualization and Quantification of Mesenchymal Cell Adipogenic Differentiation Potential with a Lineage Specific Marker
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Decreased PEDF Expression Promotes Adipogenic Differentiation through the Up-Regulation of CD36

Kuang-Tzu Huang1,2, Li-Wen Hsu3, Kuang-Den Chen4,5

  • 1Institute for Translational Research in Biomedicine, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung 83301, Taiwan. huangkt@cgmh.org.tw.

International Journal of Molecular Sciences
|December 15, 2018
PubMed

Insights

Pigment epithelium-derived factor (PEDF) negatively regulates fat cell formation (adipogenesis). Lowering PEDF increases adipocyte differentiation and lipid metabolism markers, suggesting PEDF

Area of Science:

  • Cell Biology
  • Metabolic Disorders
  • Molecular Endocrinology

Background:

  • Adipogenesis is a complex, regulated process vital for understanding obesity and metabolic diseases.
  • Pigment epithelium-derived factor (PEDF), a glycoprotein, is known for neurotrophic roles and its partnership with adipose triglyceride lipase (ATGL).

Purpose of the Study:

  • To investigate the impact of PEDF on adipogenic differentiation.
  • To elucidate the role of PEDF in regulating key adipogenesis and lipid metabolism markers.

Main Methods:

  • Utilized rat adipose-derived stem cells (AdSCs) and mouse 3T3-L1 pre-adipocytes.
  • Employed gene knockdown techniques to reduce PEDF levels during differentiation.
  • Quantified adipogenic and lipogenic markers, including ATGL and CD36, and analyzed PPARγ regulation.

Main Results:

  • PEDF knockdown led to increased levels of ATGL, CD36, and other adipogenic markers, enhancing adipocyte differentiation.
  • CD36 was identified as a regulator of proliferation and lipogenic gene expression, with its increase linked to elevated PPARγ following PEDF down-regulation.
  • Dexamethasone was found to regulate PEDF expression transcriptionally, a common inducer of adipogenesis.

Conclusions:

  • PEDF acts as a negative regulator of adipogenesis by modulating signaling intermediates like ATGL and CD36.
  • The findings suggest PEDF's critical involvement in lipid metabolism and its potential role in metabolic disorders.
  • PEDF's expression is influenced by dexamethasone, highlighting a link between glucocorticoids and adipogenesis regulation.

Keywords:
CD36adipogenic differentiationadipose triglyceride lipaseadipose-derived stem cellspigment epithelium-derived factor

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