Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[The structure of ribosome decoding center].

A P Potapov

    Molekuliarnaia Biologiia
    |July 1, 1988
    PubMed
    Summary

    A new model proposes direct interaction between ribosomes and genetic code molecules. Ribosomal protein beta-sheets may bind codon-anticodon backbones, explaining protein synthesis accuracy.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    [Health schools for patients with chronic cardiac insufficiency (innovative technologies)].

    Klinicheskaia meditsina·2010
    Same author

    The effect of mutations in EF-Tu on its affinity for tRNA as measured by two novel and independent methods of general applicability.

    Journal of biochemical and biophysical methods·2000
    Same author

    Ribosomal decoding processes at codons in the A or P sites depend differently on 2'-OH groups.

    The Journal of biological chemistry·1995
    Same author

    [Analysis of stationary kinetics of translation elongation within the framework stereospecific stabilization hypothesis of codon- anticodon complexes in a ribosome. I. Kinetic schemes of factorless elongation].

    Molekuliarnaia biologiia·1995
    Same author

    [Analysis of stationary kinetics of translation elongation within the framework of the stereospecific stabilization hypothesis of codon- anticodon complexes in a ribosome. II. Kinetic schemes in the presence of protein elongation factors and GTP].

    Molekuliarnaia biologiia·1995
    Same author

    Synergism between the GTPase activities of EF-Tu.GTP and EF-G.GTP on empty ribosomes. Elongation factors as stimulators of the ribosomal oscillation between two conformations.

    Journal of molecular biology·1994

    Area of Science:

    • Molecular Biology
    • Structural Biology
    • Bioinformatics

    Background:

    • The ribosome is central to protein synthesis, translating genetic information from messenger RNA (mRNA) into amino acid sequences.
    • Understanding the precise molecular interactions within the ribosome's decoding center is crucial for elucidating translation fidelity.
    • The current understanding of direct physical interactions between ribosomal proteins and the codon-anticodon complex remains incomplete.

    Purpose of the Study:

    • To propose a structural model for direct interaction between the ribosome and the codon-anticodon sugar-phosphate backbones.
    • To identify potential ribosomal proteins and structural motifs involved in this interaction.
    • To analyze the properties and implications of these hypothesized interactions for translation.

    Main Methods:

    • Development of a computational model for the ribosome's decoding center structure.
    • Analysis of structural complementarity between protein beta-sheets and double-stranded RNA (dsRNA) structures.
    • Bioinformatic analysis of known Escherichia coli ribosomal protein sequences for specific amino acid repeat patterns (arginine and glycine boxes).

    Main Results:

    • A model featuring a right-handed, two-stranded, antiparallel beta-sheet as the core structural element.
    • Postulation that one peptide strand interacts with the codon backbone and the second with the anticodon backbone via H-bonds.
    • Identification of specific arginine and glycine repeat sequences in E. coli ribosomal proteins (S1, S10, S11, S14, S5, L15) as potential candidates for forming these beta-sheets.

    Conclusions:

    • The proposed model suggests a direct physical mechanism for ribosome-codon-anticodon recognition involving beta-sheet structures.
    • Specific ribosomal proteins containing arginine or glycine repeat motifs are implicated in forming the decoding center's interaction surfaces.
    • This structural hypothesis provides a framework for understanding how the ribosome ensures accurate translation of the genetic code.

    Related Experiment Videos