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MicroRNA-466 inhibits osteosarcoma cell proliferation and induces apoptosis by targeting CCND1

Wei Cao1, Le Fang2, Siyong Teng3

  • 1Clinical Laboratory, Beijing Rehabilitation Hospital of Capital Medical University, Beijing 100041, P.R. China.

Experimental and Therapeutic Medicine
|December 15, 2018
PubMed

Insights

MicroRNA-466 (miR-466) acts as a tumor suppressor in osteosarcoma (OS) by inhibiting cancer cell growth and promoting apoptosis. Downregulation of miR-466 predicts poor prognosis, highlighting its potential as a therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • MicroRNA-466 (miR-466) is recognized as a tumor suppressor in various cancers.
  • Its role in osteosarcoma (OS) progression is not well-established.

Purpose of the Study:

  • To investigate the function and mechanism of miR-466 in osteosarcoma.
  • To determine if miR-466 can serve as a prognostic biomarker for OS.

Main Methods:

  • Quantitative real-time PCR to assess miR-466 expression in OS tissues and cell lines.
  • Functional assays (proliferation, cell cycle, apoptosis) to evaluate miR-466's impact on OS cells.
  • Western blot and luciferase reporter assays to identify and validate miR-466 targets.

Main Results:

  • miR-466 expression was significantly downregulated in OS tissues and cell lines, correlating negatively with OS severity.
  • Low miR-466 expression predicted poor prognosis in OS patients.
  • Overexpression of miR-466 inhibited OS cell proliferation and cell cycle progression while inducing apoptosis.
  • CCND1 was identified as a direct target of miR-466, and its restoration rescued the anti-tumor effects of miR-466.

Conclusions:

  • miR-466 suppresses osteosarcoma progression by targeting CCND1.
  • miR-466 holds potential as a promising biomarker and therapeutic target for osteosarcoma.
Keywords:
CCND1apoptosismiR-466osteosarcomaproliferation

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