Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Packaging00:58

DNA Packaging

112.5K
Overview
112.5K
Chromatin Packaging01:32

Chromatin Packaging

19.2K
Each human somatic cell contains 6 billion base pairs of DNA. Each base pair is 0.34 nm long, meaning each diploid cell contains a staggering 2 meters of DNA. This long DNA strand is packed inside a nucleus measuring only 10-20 microns in diameter with the help of specialized DNA-binding proteins called histones. Together they form a compact DNA-protein complex called chromatin. The chromatin is further compacted into higher-order structures. The highest level of compaction is achieved during...
19.2K
Chromatin Packaging02:21

Chromatin Packaging

22.1K
Each human somatic cell contains 6 billion base-pairs of DNA. Each base-pair is 0.34 nm long, which means that each diploid cell contains a staggering 2 meters of DNA. How is such a long DNA strand packed inside a nucleus measuring only 10 - 20 microns in diameter? 
The chromatin
In combination with specialized DNA binding protein called Histones, the DNA double helix forms a compact DNA: protein complex called chromatin. The chromatin itself is further compacted into higher-order...
22.1K
Chromatin Packaging02:21

Chromatin Packaging

9.7K
9.7K
Encoding01:19

Encoding

846
Information enters the brain through encoding, which is the input of information into the memory system. Once sensory information is received from the environment, the brain labels or codes it. The information is then organized with similar information and connected to existing concepts. Encoding occurs through automatic processing and effortful processing.
Automatic processing involves the encoding of details like time, space, frequency, and the meaning of words, usually done without conscious...
846
Animal Mitochondrial Genetics02:59

Animal Mitochondrial Genetics

9.2K
Among all the organelles in an animal cell, only mitochondria have their own independent genomes. Animal mitochondrial DNA is a double-stranded, closed-circular molecule with around 20,000 base pairs. Mitochondrial DNA is unique in that one of its two strands, the heavy, or H, -strand is guanine rich, whereas the complementary strand is cytosine rich and called the light, or L, -strand. Compared to nuclear DNA, mitochondrial DNA has a very low percentage of non-coding regions and is marked by...
9.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Development and validation of a pseudotyped virus neutralization assay for quantification of anti-Lassa virus neutralizing antibodies.

Frontiers in immunology·2026
Same author

Antibody format matters: A comparative analysis of VHH and scFv domains reveals superior in vivo CAR T cell function with VHH domains.

Molecular therapy : the journal of the American Society of Gene Therapy·2026
Same author

Optimization of a parallel CAR for B-cell lymphoma via ITAM attenuation and target specificity validation.

Clinical and experimental immunology·2026
Same author

RNA activation as a precision dosing modality: MTL-CEBPA for controlled enzyme elevation in MPS I-H.

Frontiers in medicine·2026
Same author

A comparison of two manufacturing methods in the phase I COBALT study of CD19CAR T for LBCL.

Molecular therapy. Advances·2026
Same author

Evaluating the use of the first WHO International Standard for anti-SARS-CoV-2 immunoglobulin to harmonise the readouts between commercial SARS-CoV-2 binding antibody tests: the HARMONY multicentre calibration study.

The Lancet. Microbe·2026

Related Experiment Video

Updated: Feb 1, 2026

A Tetracycline-regulated Cell Line Produces High-titer Lentiviral Vectors that Specifically Target Dendritic Cells
16:04

A Tetracycline-regulated Cell Line Produces High-titer Lentiviral Vectors that Specifically Target Dendritic Cells

Published on: June 19, 2013

15.1K

Lentiviral Vector Purification Using Genetically Encoded Biotin Mimic in Packaging Cell.

Leila Mekkaoui1, Farhaan Parekh1, Ekaterini Kotsopoulou2

  • 1UCL Cancer Institute, University College London, Paul O'Gorman Building, 72 Huntley Street, London WC1E 6BT, UK.

Molecular Therapy. Methods & Clinical Development
|December 15, 2018
PubMed
Summary

Researchers developed a novel one-step purification method for lentiviral vectors (LVs) using a biotin-streptavidin system. This technique significantly improves yield and purity, addressing a key bottleneck in LV applications.

Keywords:
affinity chromatographybiotin mimiccompetitive elutionlentiviral vectorspurificationstreptavidinsynthetic peptide

More Related Videos

An Efficient In Vitro Transposition Method by a Transcriptionally Regulated Sleeping Beauty System Packaged into an Integration Defective Lentiviral Vector
10:13

An Efficient In Vitro Transposition Method by a Transcriptionally Regulated Sleeping Beauty System Packaged into an Integration Defective Lentiviral Vector

Published on: January 12, 2018

9.4K
Production of Lentiviral Vectors for Transducing Cells from the Central Nervous System
08:46

Production of Lentiviral Vectors for Transducing Cells from the Central Nervous System

Published on: May 24, 2012

37.0K

Related Experiment Videos

Last Updated: Feb 1, 2026

A Tetracycline-regulated Cell Line Produces High-titer Lentiviral Vectors that Specifically Target Dendritic Cells
16:04

A Tetracycline-regulated Cell Line Produces High-titer Lentiviral Vectors that Specifically Target Dendritic Cells

Published on: June 19, 2013

15.1K
An Efficient In Vitro Transposition Method by a Transcriptionally Regulated Sleeping Beauty System Packaged into an Integration Defective Lentiviral Vector
10:13

An Efficient In Vitro Transposition Method by a Transcriptionally Regulated Sleeping Beauty System Packaged into an Integration Defective Lentiviral Vector

Published on: January 12, 2018

9.4K
Production of Lentiviral Vectors for Transducing Cells from the Central Nervous System
08:46

Production of Lentiviral Vectors for Transducing Cells from the Central Nervous System

Published on: May 24, 2012

37.0K

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Gene Therapy

Background:

  • Lentiviral vectors (LVs) are increasingly used in research and clinical settings.
  • Current LV purification methods are inefficient, costly, and yield low recovery rates, hindering widespread application.

Purpose of the Study:

  • To develop a streamlined, one-step purification process for lentiviral vectors (LVs).
  • To enhance LV purity and yield using a novel biotin-streptavidin-based system.

Main Methods:

  • Genetically engineered HEK293T cells to display a biotin-mimicking peptide (cTag8) on LVs during budding.
  • Utilized streptavidin magnetic beads for LV capture via cTag8.
  • Employed competitive elution with biotin to release purified LVs.

Main Results:

  • Achieved overall LV yields of ≥60% with high purity.
  • Demonstrated >3-log reduction in DNA and >2-log reduction in host cell proteins.
  • Preliminary scalability testing using monolith affinity chromatography showed a 20% yield.

Conclusions:

  • The developed one-step purification method offers high yield and purity for LVs.
  • This approach effectively removes contaminants and is potentially scalable for clinical applications.
  • The cTag8 system provides a valuable tool for advancing LV research and therapeutic use.