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Quantification of Infectious Sendai Virus Using Plaque Assay.
Narihito Tatsumoto1, Takamasa Miyauchi1, Moshe Arditi2,3
1Department of Pathology and Laboratory Medicine, Cedars-Sinai Medical Center, Los Angeles, USA.
Bio-Protocol
|December 15, 2018
Summary
This study details a Sendai virus (SeV) plaque assay protocol to quantify plaque-forming units (PFU). This method is crucial for accurate viral stock and experimental sample analysis in immunology research.
Area of Science:
- Virology
- Immunology
Background:
- Sendai virus (SeV), an enveloped RNA virus from the Paramyxoviridae family, serves as a model for studying infectious pathomechanisms.
- Accurate quantification of SeV is vital for experimental design and analysis, particularly in immunology and IgA nephropathy models.
- Common quantification methods include plaque-forming units (PFU) and hemagglutination (HA) titers.
Purpose of the Study:
- To describe a reliable protocol for determining Sendai virus plaque-forming units (PFU).
- To provide a method for quantifying SeV in both viral stocks and experimental samples.
Main Methods:
- A plaque assay protocol using LLC-MK2 cells (rhesus monkey kidney cell line).
- Utilization of Guinea pig red blood cells in the assay.
- Determination of viral titers in plaque-forming units (PFU).
Main Results:
- The described plaque assay method effectively quantifies Sendai virus PFU.
- The protocol is applicable to viral stock assessment and analysis of experimental harvests.
Conclusions:
- The presented Sendai virus plaque assay offers a standardized method for PFU determination.
- This protocol supports accurate viral quantification essential for immunological studies involving SeV.
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