Related Experiment Video
Updated: Feb 1, 2026

Plaquing of Herpes Simplex Viruses
Published on: November 5, 2021
Quantification of Infectious Sendai Virus Using Plaque Assay
Narihito Tatsumoto1, Takamasa Miyauchi1, Moshe Arditi2,3
1Department of Pathology and Laboratory Medicine, Cedars-Sinai Medical Center, Los Angeles, USA.
Abstract:
Sendai virus (SeV) is an enveloped, single-stranded RNA virus of the family Paramyxoviridae. SeV is a useful tool to study its infectious pathomechanism in immunology and the pathomechanism of a murine model of IgA nephropathy. Virus quantification is essential not only to determine the original viral titers for an appropriate application, but also to measure the viral titers in samples from the harvests from experiments. There are mainly a couple of units/titers for Sendai viral quantification: plaque-forming units (PFU) and hemagglutination (HA) titer. Of these, we here describe a protocol for Sendai virus plaque assay to provide PFU using LLC-MK2 cells (a rhesus monkey kidney cell lines) and Guinea pig red blood cells. This traditional protocol enables us to determine Sendai virus PFU in viral stock as well as samples from your experiments.
Related Concept Videos
What are Viruses?
Introduction to Virus
Viruses of Archaea
Viruses with RNA Genomes
Agonism and Antagonism: Quantification
To quantify these effects, researchers use a dose-response curve, which provides valuable information about the potency and efficacy of a drug. Potency refers to...
Intracellular Movement of Viruses and Bacteria

