[In vitro activity of beta-lactams, clindamycin and metronidazole on Clostridium difficile]

In one year and a half period 47 Clostridium difficile were isolated from stool samples. Isolates with questionable morphologic features were identified using sugars fermentation, gaz liquid chromatography (GLC). Cytotoxin assay was performed on Mac Coy cells. The comparative susceptibilities to amoxicillin-clavulanic acid (AMC), cefotetan (CTT), cefoxitin (FOX), cefotaxime (CTX), latamoxef (MOX), mezlocillin (MEZ), piperacillin (PIP), clindamycin (CLIN) and metronidazole was tested using minimal inhibition concentration (MIC) determination on Wilkins-Chalgren blood agar. CLIN, FOX, CTX, MOX were inactive, CTT is moderately inactive. All strains were susceptible to AMX, MEZ, PIP and metronidazole.