Related Experiment Video

Updated: Feb 1, 2026

Prostaglandin Extraction and Analysis in Caenorhabditis elegans
08:50

Prostaglandin Extraction and Analysis in Caenorhabditis elegans

Published on: June 25, 2013

20.1K

Naproxen attenuates osteoarthritis progression through inhibiting the expression of prostaglandinl-endoperoxide

Cuijiang Wang1, Fei Wang1, Fen Lin2

  • 1Department of Pain Management, Linyi People's Hospital, Linyi, Shandong, China.

Journal of Cellular Physiology
|December 15, 2018
PubMed
Abstract

Insights

Naproxen treatment suppresses prostaglandin-endoperoxide synthase 1 (PTGS1) in osteoarthritis (OA) synovial cells. This reduces OA cell migration and invasion while promoting apoptosis, offering a potential therapeutic strategy for OA.

Area of Science:

  • Biomedical research
  • Molecular biology
  • Pharmacology

Background:

  • Osteoarthritis (OA) is a degenerative joint disease characterized by cartilage breakdown and synovial inflammation.
  • Identifying molecular targets for OA treatment is crucial for developing effective therapies.

Purpose of the Study:

  • To investigate the therapeutic effects of naproxen on osteoarthritis.
  • To identify the biological target of naproxen in OA synovial cells.

Main Methods:

  • Messenger RNA microarray analysis to identify differentially expressed genes (DEGs) in OA synovial tissues.
  • Weighted gene coexpression network analysis to determine hub genes.
  • Quantitative real-time polymerase chain reaction and western blot to validate PTGS1 expression.
  • Transwell assays and in vivo mouse models (anterior cruciate ligament transection) to assess cell migration, invasion, and apoptosis.

Main Results:

  • Prostaglandin-endoperoxide synthase 1 (PTGS1) was identified as a hub gene overexpressed in OA synovial cells.
  • Naproxen treatment significantly downregulated PTGS1 expression in OA synovial cells.
  • Naproxen inhibited OA synoviocyte migration and invasion while promoting apoptosis.
  • Naproxen influenced the expression of other OA-related genes, including MMP-13 and COX-2.

Conclusions:

  • Naproxen effectively suppresses PTGS1 expression in OA synovial cells.
  • Naproxen demonstrates therapeutic potential by inhibiting OA synoviocyte migration/invasion and promoting apoptosis.
Keywords:
naproxenosteoarthritis (OA)prostaglandin-endoperoxide synthase 1 ( PTGS1)weighted gene coexpression network analysis (WGCNA)

More Related Videos

Synovial Fluid Analysis to Identify Osteoarthritis07:51

Synovial Fluid Analysis to Identify Osteoarthritis

Published on: October 20, 2022

6.3K
Assessing Hepatic Metabolic Changes During Progressive Colonization of Germ-free Mouse by 1H NMR Spectroscopy07:54

Assessing Hepatic Metabolic Changes During Progressive Colonization of Germ-free Mouse by 1H NMR Spectroscopy

Published on: December 15, 2011

13.5K

Related Concept Videos

Transcription Attenuation in Prokaryotes02:42

Transcription Attenuation in Prokaryotes

Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure.  Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
18.4K
ATP Synthase: Structure01:18

ATP Synthase: Structure

ATP synthase or ATPase is among the most conserved proteins found in bacteria, mammals, and plants. This enzyme can catalyze a forward reaction in response to the electrochemical gradient, producing ATP from ADP and inorganic phosphate. ATP synthase can also work in a reverse direction by hydrolyzing ATP and generating an electrochemical gradient. Different forms of ATP synthases have evolved special features to meet the specific demands of the cell. Based on their specific feature, ATP...
15.5K
Feedback Inhibition00:46

Feedback Inhibition

Biochemical reactions are occurring constantly in cells, converting starting substances to different products, usually with the help of enzymes that speed the reactions. Without enzymes, it would take far too long for most reactions to occur to be useful to the cell!
57.1K
ATP Synthase: Mechanism01:48

ATP Synthase: Mechanism

In animals, the mitochondrial F1F0 ATP synthase is the key protein that synthesizes ATP molecules through a complex catalytic mechanism. While the nuclear genome encodes the majority of ATP synthase subunits, the mitochondrial genome encodes some of the enzyme's most critical components. The formation of this multi-subunit enzyme is a complex multi-step process regulated at the level of transcription, translation, and assembly. Defects in one or more of these steps can result in decreased...
17.1K
What is Gene Expression?01:42

What is Gene Expression?

Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
196.7K
Tumor Progression02:07

Tumor Progression

Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
7.4K