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Updated: Feb 1, 2026

Genotypic Inference of HIV-1 Tropism Using Population-based Sequencing of V3
Published on: December 27, 2010
Development of an HIV-1 integrase genotyping assay for HIV-1 subtype AE
Yang Liu1, Nicolas Margot1, Kathryn M Kitrinos1
1Clinical Virology, Gilead Sciences, Inc, Foster City, California.
Abstract:
An human immunodeficiency virus (HIV)-1 integrase (IN) genotyping assay was developed to evaluate clinical samples from patients infected with HIV-1 subtype AE, a subtype highly prevalent in Asia. The HIV-1 IN gene was amplified from plasma-derived HIV-1 viral RNA via reverse transcription polymerase chain reaction followed by population sequencing. Assay sensitivity was also evaluated using serially diluted plasma samples. The genotyping assay successfully amplified the IN gene from patient plasma samples with HIV-1 RNA as low as 500 copies/mL. This assay is suitable for IN genotyping to identify IN drug resistance mutations in HIV-1 patients harboring subtype AE virus.
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