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Updated: Feb 1, 2026

Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization IR-MALDESI
Published on: March 24, 2016
Gold-nanoparticle-coated magnetic beads for concentration and ionization of analytes for laser desorption/ionization
Moon-Ju Kim1, Jong-Min Park1, Joo-Yoon Noh1
1Department of Materials Science and Engineering, Yonsei University, 134 Shinchon-dong, Seodaemun-gu, Seoul, 120-749, Korea.
Rationale:
Magnetic particles coated with gold nanoparticles (Au NPs) (Au-MAGs) were developed and used (1) for sample concentration and (2) as a solid matrix for laser adsorption/desorption mass spectrometry (LDI-MS).
Methods:
The Au-MAGs were prepared by (1) coating polystyrene on iron oxide NPs (PS-MNPs), (2) coating poly-l-lysine on the PS-MNPs (PLL-coated PS-MNPs), and (3) coating negatively charged Au NPs on the PLL-coated PS-MNPs (Au-MAGs).
Results:
The Au-MAGs were used to concentrate the target analyte by means of electrostatic interactions between the positively charged GHP9 and the negatively charged Au-MAGs as well as selective interactions (such as gold-sulfur interactions) between glutathione (GSH) and Au-MAGs. Then, the concentrated analyte could be ionized for LDI-MS.
Conclusions:
The Au-MAGs were demonstrated (1) to concentrate the target analyte in a sample solution, tested by electrostatic interactions and selective interactions between gold and sulfur and (2) to ionize the concentrated analyte for LDI-MS.
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