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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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At room temperature, the chair conformer of cyclohexane undergoes rapid ring flipping between two equivalent chair conformers at a rate of approximately 105 times per second. These two chair conformers are in equilibrium. The rapid ring flipping results in the interconversion of the axial proton to an equatorial proton and an equatorial to the axial proton. Such interconversions are too rapid and cannot be detected on the NMR timescale. Hence, the NMR spectrometer cannot distinguish between the...
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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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Total Internal Reflection Fluorescence Microscopy01:05

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Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
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Immunoglobulin-like Cell Adhesion Molecules01:31

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Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
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Related Experiment Video

Updated: Feb 1, 2026

Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking
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Small-Molecule Fluorescent Probes for Live-Cell Super-Resolution Microscopy.

Lu Wang1, Michelle S Frei1,2, Aleksandar Salim1,2

  • 1Department of Chemical Biology , Max Planck Institute for Medical Research , Jahnstrasse 29 , 69120 Heidelberg , Germany.

Journal of the American Chemical Society
|December 15, 2018
PubMed
Summary

Small-molecule fluorescent probes are crucial for live-cell super-resolution microscopy, enabling visualization of dynamic cellular processes. Overcoming challenges in probe development is key to advancing this powerful imaging technique.

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Related Experiment Videos

Last Updated: Feb 1, 2026

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Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes
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Super-Resolution Live Cell Imaging of Subcellular Structures
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Area of Science:

  • Cellular and Molecular Imaging
  • Biophysics
  • Chemical Biology

Background:

  • Super-resolution fluorescence microscopy offers nanoscale visualization of biomolecules and cellular structures.
  • Live-cell imaging with these techniques allows studying dynamic biological processes at high resolution.
  • A major limitation is the lack of suitable fluorescent probes for specific biomolecule labeling.

Purpose of the Study:

  • To discuss the significance of small-molecule fluorescent probes in live-cell super-resolution microscopy.
  • To highlight challenges in generating effective probes for this application.
  • To review recent advancements in labeling strategies and probe properties.

Main Methods:

  • Review of current literature on small-molecule fluorescent probes.
  • Analysis of chemical and spectroscopic requirements for live-cell imaging probes.
  • Discussion of labeling strategies and their impact on probe performance.

Main Results:

  • Small-molecule probes are essential for overcoming current limitations in live-cell super-resolution microscopy.
  • Development requires addressing specific chemical and spectroscopic challenges.
  • Emerging trends focus on improved labeling strategies and probe design.

Conclusions:

  • Small-molecule fluorescent probes play a vital role in advancing live-cell super-resolution microscopy.
  • Continued innovation in probe development is necessary to fully exploit the potential of these imaging techniques.
  • This perspective outlines key areas for future research in probe design and application.