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Three monophyletic clusters in Retortamonas species isolated from vertebrates
Joko Hendarto1, Tetsushi Mizuno2, Anggi P N Hidayati3
1Department of Parasitology, Graduate School of Medical Sciences, Kanazawa University, Kanazawa 920-8640, Japan; Department of Public Health and Preventive Medicine, Faculty of Medicine, Hasanuddin University, Makassar 90245, Indonesia.
Parasitology International
|December 15, 2018
Summary
Molecular diversity of Retortamonas spp. was analyzed using 18S rRNA gene sequences. Three distinct clusters were identified among vertebrate isolates, with a specific haplotype indicating potential zoonotic transmission in mammals.
Area of Science:
- Molecular Parasitology
- Microbial Phylogenetics
- Zoonotic Disease Research
Background:
- Retortamonas spp. are recognized intestinal parasites in diverse hosts, including humans.
- Limited understanding exists regarding the intra-genus molecular diversity of Retortamonas spp.
- Elucidating this diversity is crucial for understanding its epidemiology and host-parasite interactions.
Purpose of the Study:
- To investigate the molecular diversity within the Retortamonas genus using the 18S ribosomal RNA (rRNA) gene.
- To analyze haplotypes from various host organisms, including humans, pigs, dogs, goats, water buffalos, cattle, rats, and chickens.
- To explore potential phylogenetic relationships and zoonotic transmission patterns.
Main Methods:
- Sequencing and analysis of the 18S small subunit ribosomal RNA locus (1836-1899 bp) from Retortamonas spp. isolates.
- Phylogenetic and network analyses were performed on obtained haplotypes.
- Comparison with reference sequences from non-human mammals, amphibians, and insects.
Main Results:
- Phylogenetic analysis revealed three statistically supported clusters among vertebrate-isolated Retortamonas haplotypes.
- Assemblage B, encompassing mammalian and chicken genotypes, included human isolates and may represent Retortamonas intestinalis.
- A specific haplotype found across multiple mammalian hosts suggests potential zoonotic transmission pathways.
Conclusions:
- The study elucidates the molecular diversity of Retortamonas spp., identifying distinct assemblages.
- Assemblage B, containing human isolates, highlights the potential for zoonotic transmission among various mammals.
- Genotyping classification provides a foundation for understanding the molecular epidemiology of Retortamonas, particularly concerning human and animal health.
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