DEPArray™ system: An automatic image-based sorter for isolation of pure circulating tumor cells.
Mariano Di Trapani1, Nicolò Manaresi1, Gianni Medoro1
1Menarini Silicon Biosystems S.p.A, Bologna, Italy.
Summary
Analyzing rare circulating tumor cells (CTCs) is crucial for cancer monitoring. The DEPArray™ technology enables precise isolation of single CTCs for genomic analysis, improving diagnostic accuracy.
Area of Science:
- Biomedical Engineering
- Molecular Biology
- Oncology
Background:
- Circulating tumor cells (CTCs) are shed by invasive tumors into the bloodstream.
- CTC analysis offers a noninvasive method for predicting and monitoring therapeutic responses.
- Current enrichment methods often include contaminating normal cells, necessitating further purification for genetic analysis.
Purpose of the Study:
- To introduce and describe the DEPArray™ technology for precise isolation of single CTCs.
- To enable reliable downstream genetic analysis of CTCs for tumor characterization.
- To advance the noninvasive monitoring of cancer and therapeutic efficacy.
Main Methods:
- Utilized DEPArray™ technology, a microchip-based digital sorter.
- Combined microfluidic and microelectronic principles for precise cell manipulation.
- Employed image-based sorting for the isolation of single CTCs.
- Integrated DEPArray™ with Next Generation Sequencing (NGS) for genetic analysis.
Main Results:
- Demonstrated the capability of DEPArray™ for precise, image-based isolation of single CTCs.
- Enabled the purification of CTCs, removing contaminating normal cells.
- Facilitated downstream genetic analysis of isolated single CTCs.
- Showcased the potential for comprehensive genomic profiling of tumors via CTCs.
Conclusions:
- DEPArray™ technology provides a robust solution for isolating pure single CTCs.
- This technology enhances the reliability of genomic analysis from rare CTCs.
- DEPArray™ significantly advances the potential of CTCs as a noninvasive tool in oncology.
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