Related Experiment Video
Updated: Feb 1, 2026

A Method for Targeted 16S Sequencing of Human Milk Samples
Published on: March 23, 2018
Comparison of macronutrient content in human milk measured by mid-infrared human milk analyzer and reference methods
Francesca Giuffrida1, Sean Austin2, Denis Cuany2
1Nestlé Research, Vers-chez-les Blanc, P.O. Box 44, 1000, Lausanne 26, Switzerland. francesca.giuffrida@rdls.nestle.com.
Objective:
The study aims at evaluating mid-infrared human milk analyzer (HMA) accuracy and precision, in human milk (HM).
Study Design:
Röse-Gottlieb, high-performance anion exchange chromatography-pulsed amperometric detection (HPAEC-PAD), Kjeldahl and amino acid analysis (AA) were selected as references for total fat, lactose and total protein determination.
Results:
No significant difference was observed in lactose content between HMA and HPAEC-PAD. Significant differences were observed in fat and protein content between HMA and reference methods. However, the difference in fat content was lower than 12%, and therefore within the variability declared by supplier. For protein determination, the BCA protein assay was selected. No significant differences were observed in total protein content measured by BCA assay, Kjeldahl and AA methods.
Conclusions:
HMA was reliable for the quantification of total fat and lactose content, but not for total protein one. The latter was measured by BCA assay, which yielded comparable results to Kjeldahl and AA methods.
Related Concept Videos
Measurement of Air Content in Concrete
The pressure method,...
Biodiversity and Human Values
The Sense of Self: Reflected Self-Appraisal and Social Comparison
Renal Drug Clearance: Comparison Between Renal Excretion Methods
Renal clearance is often associated with the renal glomerular filtration rate (GFR), which represents the rate at which plasma is filtered through the glomeruli in the kidney. When drug reabsorption is minimal and there is no active secretion, renal clearance is closely related to the...
Inertial Frames of Reference
Non-inertial Frames of Reference

