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ADAM28 dramatically regulates the biological features of human gingival fibroblasts

Zheng Zhao1, Jie Li2, Xiu-Na Ding2

  • 1Qingdao Stomatological Hospital, No.17 De-xian Road, Qingdao, 266000, Shandong, People's Republic of China. zhaozheng170@163.com.

Odontology
|December 16, 2018
PubMed

Insights

ADAM28 (a disintegrin and metalloproteinase 28) regulates human gingival fibroblast (HGF) growth, differentiation, and apoptosis. Blocking ADAM28 inhibits proliferation and enhances differentiation, crucial for root development.

Area of Science:

  • Cell Biology
  • Biochemistry
  • Developmental Biology

Background:

  • ADAM28 is a metalloproteinase with roles in tissue remodeling.
  • Its specific function in human gingival fibroblasts (HGFs) and root development is not fully understood.

Purpose of the Study:

  • To investigate the effects of ADAM28 on HGF proliferation, differentiation, and apoptosis.
  • To elucidate the potential mechanism involving ADAM28 in root development.

Main Methods:

  • ADAM28 expression was modulated using antisense (AS-ODN) and sense (S-ODN) oligodeoxynucleotides in HGFs.
  • Cell proliferation was assessed by MTT assay and cell-cycle analysis.
  • Apoptosis was measured by Annexin V/PI staining, and differentiation by alkaline phosphatase (ALP) activity.
  • Expression of key proteins (Cbfα1, CEMP1, OPN, DMP1) was analyzed via immunocytochemistry and Western blotting.

Main Results:

  • ADAM28 AS-ODN significantly reduced ADAM28 expression, while S-ODN increased it.
  • Inhibition of ADAM28 suppressed HGF proliferation and induced apoptosis.
  • Conversely, ADAM28 overexpression promoted proliferation and inhibited apoptosis.
  • ADAM28 AS-ODN treatment decreased the expression of Cbfα1 and CEMP1, indicating a positive correlation between ADAM28 and these differentiation markers.

Conclusions:

  • ADAM28 plays a critical role in regulating HGF proliferation, differentiation, and apoptosis.
  • ADAM28 influences key markers of cementoblast differentiation, suggesting its involvement in root development.
  • ADAM28 acts as a potential regulator in the complex processes of tooth root formation.
Keywords:
ADAM28ApoptosisDifferentiationGingival fibroblastsProliferation

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