Related Experiment Video
Updated: Feb 1, 2026
12:27
Quantifying the Cytotoxicity of Staphylococcus aureus Against Human Polymorphonuclear Leukocytes
Published on: January 3, 2020
6.8K
Draft genome sequence of a human-associated streptogramin-resistant Staphylococcus aureus
Sushim K Gupta1, Poonam Sharma1, John B Barrett1
1Bacterial Epidemiology and Antimicrobial Resistance Research Unit, Richard B. Russell Research Center, US National Poultry Research Center, USDA-ARS, Athens, GA 30605, USA.
Journal of Global Antimicrobial Resistance
|December 16, 2018
Summary
Genomic analysis of Staphylococcus aureus strain CIP108540 revealed multiple antimicrobial resistance genes. This study enhances understanding of the bacterial resistome and informs strategies against infections caused by this pathogen.
Area of Science:
- Microbiology
- Genomics
- Infectious Diseases
Background:
- Staphylococcus aureus is a major cause of hospital and community infections.
- Antibiotic resistance, particularly to macrolide-lincosamide-streptogramin (MLS) drugs, is a growing concern.
- Previous studies confirmed streptogramin resistance in S. aureus strain CIP108540, but the full spectrum of MLS resistance genes was unknown.
Purpose of the Study:
- To analyze the complete genome sequence of S. aureus strain CIP108540.
- To identify all antimicrobial resistance genes present in this strain, focusing on MLS resistance.
- To contribute to understanding the genetic basis of antibiotic resistance in S. aureus.
Main Methods:
- Whole-genome sequencing of S. aureus CIP108540 using Illumina MiSeq technology.
- De novo assembly of sequencing reads utilizing the A5-miseq pipeline.
- Bioinformatic analysis to identify antimicrobial resistance genes.
Main Results:
- The draft genome of S. aureus CIP108540 has a size of 3,014,273 bp with a GC content of 32.72%.
- The genome contains 3,063 predicted coding sequences and 59 tRNAs.
- Several genes conferring resistance to various antibiotics, including MLS agents, were identified.
Conclusions:
- The released draft genome sequence of S. aureus CIP108540 provides crucial insights into its genetic composition.
- This genomic data is valuable for understanding the strain's resistome.
- The findings support further research into the mechanisms of antibiotic resistance in S. aureus.
Related Concept Videos
02:02Genomics
40.6K
Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
40.6K
03:21Genome Size and the Evolution of New Genes
9.1K
While every living organism has a genome of some kind (be it RNA, or DNA), there is considerable variation in the sizes of these blueprints. One major factor that impacts genome size is whether the organism is prokaryotic or eukaryotic. In prokaryotes, the genome contains little to no non-coding sequence, such that genes are tightly clustered in groups or operons sequentially along the chromosome. Conversely, the genes in eukaryotes are punctuated by long stretches of non-coding sequence.
9.1K
02:30Genomic Imprinting and Inheritance
37.2K
Diploid organisms inherit genetic material through chromosomes from both parents. Copies of the same gene are known as alleles. In most cases, both alleles are simultaneously expressed and allow various cellular processes to function optimally. If one of the alleles is missing or mutated, the expression of the other allele can compensate; however, this is not true for all genes.
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...
37.2K
02:16Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes
16.0K
The present-day mitochondrial and chloroplast genomes have retained some of the characteristics of their ancestral prokaryotes and also have acquired new attributes during their evolution within eukaryotic cells. Like prokaryotic genomes, mitochondrial and chloroplast genomes neither bind with histone-like proteins nor show complex packaging into chromosome-like structures, as observed in eukaryotes. Unlike mitotic cell divisions observed in eukaryotic cells, mitochondria and chloroplasts...
16.0K
02:02Cis-regulatory Sequences
11.8K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
11.8K
01:22Resistivity
4.6K
When a voltage is applied to a conductor, an electrical field is generated, and charges in the conductor feel the force due to the electrical field. The current density that results depends on the electrical field and the properties of the material. In some materials, including metals at a given temperature, the current density is approximately proportional to the electrical field. In these cases, the current density can be modeled as:
4.6K