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Updated: Feb 1, 2026

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
Published on: March 20, 2015
In situ study of EDC/NHS immobilization on gold surface based on attenuated total reflection surface-enhanced
Tien-Chun Tsai1, Chia-Wei Liu2, Yi-Chen Wu1
1Department of Biomedical Engineering, National Cheng Kung University, Tainan, 70101, Taiwan.
Abstract:
In this study, the time-dependent reaction between 11-mercaptoundecanoic acid (11-MUA) and 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-hydroxysuccinimide (EDC/NHS) is precisely characterized using surface enhanced infrared absorption spectroscopy (SEIRAS). According to the high correlation between the spectral results of SEIRAS and the electrochemical behavior, it strongly demonstrates that the EDC/NHS reaction would be obviously interfered by phosphate ions in the neutral pH condition (pH = 7.0).
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