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Updated: Feb 1, 2026

Activation and Conjugation of Soluble Polysaccharides using 1-Cyano-4-Dimethylaminopyridine Tetrafluoroborate CDAP
Published on: June 14, 2021
Physicochemical properties, immunostimulatory activity of the Lachnum polysaccharide and polysaccharide-dipeptide
Guohua Hou1, Xue Chen1, Jinglei Li1
1Microbial Resources and Application Laboratory, School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China.
Abstract:
The physicochemical properties and the immunoregulatory actions in vitro of an exopolysaccharide from Lachnum (LEP) and its conjugation with a dipeptide (LEP-RH) were investigated aiming to improve their functional characteristics. The structure characteristic of the LEP and LEP-RH were determined via FT-IR and NMR. The physicochemical properties were evaluated by scanning electron microscopy (SEM), rheometer, and differential scanning calorimeter (DSC). SEM results showed that LEP-RH had a rough surface and relatively loose distribution that different from LEP. Rheological studies of LEP and LEP-RH at the same concentration indicated that LEP and LEP-RH have similar shear-thinning behaviors and gel-like structures, while LEP-RH has a better thermal stability than LEP. Bioassay results showed that treatment with the higher dosage (200 μg/mL) of LEP and LEP-RH stimulated the proliferation, cytokine secretion (IL-2, IL-6 and TNF-α) of RAW264.7 macrophages.
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