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Updated: Feb 1, 2026
05:19
A New Technique for Treating Low-risk Prostate Cancer—Super Active Surveillance
Published on: November 7, 2025
845
Rapid Next-Generation Sequencing Method for Prediction of Prostate Cancer Risks
Viacheslav Y Fofanov1, Kinnari Upadhyay2, Alexander Pearlman2
1School of Informatics, Computing and Cyber Systems, Northern Arizona State University, Flagstaff, Arizona.
The Journal of Molecular Diagnostics : JMD
|December 17, 2018
Summary
A new sequencing assay accurately predicts prostate cancer metastasis using genomic copy number alterations. This tool aids active surveillance decisions for men with low-risk disease, improving quality of life.
Area of Science:
- Oncology
- Genomics
- Molecular Diagnostics
Background:
- Prostate cancer is a leading cause of male cancer deaths.
- Active surveillance is used for low-risk disease, but requires managing risk of aggressive cancer.
- Predicting metastatic potential from biopsy is crucial for treatment selection.
Purpose of the Study:
- To develop and validate a novel sequencing assay for predicting prostate cancer metastatic potential.
- To enable accurate risk stratification for active surveillance decisions.
Main Methods:
- Developed a targeted postligation amplification sequencing approach: the next-generation copy number alteration assay.
- Interrogated 902 genomic sites within 194 regions relevant to metastasis potential score (MPS) calculation.
- Validated assay performance on cell lines, surgical samples, and biopsy samples.
Main Results:
- The assay demonstrated technical reproducibility, robustness to low input DNA, and high accuracy.
- Performance was excellent across various sample types, including prostate cancer biopsies.
- The assay effectively estimates copy number alteration events for MPS calculation.
Conclusions:
- The next-generation copy number alteration assay is a validated tool for predicting prostate cancer metastatic potential.
- This assay represents a significant advancement for integrating sequencing into prostate cancer evaluation and active surveillance.
- Enables better management of men with low-risk prostate cancer, potentially preserving quality of life.
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