Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Zygotic Development And Stem Cell Formation01:10

Zygotic Development And Stem Cell Formation

6.7K
The development of all multicellular organisms starts with the fusion of haploid cells called sperm and egg to form a diploid zygote. A zygote is a totipotent cell that can develop into a complete organism. The zygote undergoes cell division or cleavage to form an 8-cell mass. Until this stage, the cells are spherical, loosely attached, and remain totipotent. Totipotent cells are capable of developing both the embryonic and the extraembryonic tissues. However, as they continue to divide, they...
6.7K
Sensitivity, Specificity, and Predicted Value01:13

Sensitivity, Specificity, and Predicted Value

1.3K
In healthcare diagnostics, laboratory tests play a crucial role in identifying and diagnosing a wide range of medical conditions. However, interpreting test results is not always straightforward. An abnormal test result does not always confirm the presence of a disease, just as a normal result does not guarantee its absence. To assess the reliability of these diagnostic tools, healthcare practitioners rely on two key statistical indicators: sensitivity and specificity.
Sensitivity is the...
1.3K
Sustainable Development01:43

Sustainable Development

15.1K
As the human population continues to grow and use resources, we must be mindful of our planet’s natural limits. Sustainable development provides a pathway to maintain and improve human life now while also ensuring that future generations will have the resources that they need. The long-term success of sustainability efforts rests on understanding the interplay between human actions and ecological systems.
15.1K
Sputum Studies II: Culture and Sensitivity01:20

Sputum Studies II: Culture and Sensitivity

1.3K
Description
Sputum culture and sensitivity is a medical procedure used to diagnose bacterial infections in the respiratory tract and select the most appropriate antibiotics for treatment. This process involves analyzing sputum samples of thick and opaque secretions produced in the lungs and airways. These samples are collected from patients and then sent to the laboratory for analysis.
The test can identify various pathogens responsible for respiratory infections, including Streptococcus,...
1.3K
Fruit Development, Structure, and Function01:58

Fruit Development, Structure, and Function

25.2K
Fruits form from a mature flower ovary. As seeds develop from the ovules contained within, the ovary wall undergoes a series of complex changes to form fruit. In some fruits, such as soybeans, the ovary wall dries; in other fruits, such as grapes, it remains fleshy. In some cases, organs other than the ovary contribute to fruit formation; such fruits are called accessory fruits.
25.2K
Urine Studies II: Urine Culture and Sensitivity Test01:26

Urine Studies II: Urine Culture and Sensitivity Test

1.1K
A urine culture and sensitivity test is a diagnostic procedure used to identify urinary tract bacterial infections and determine the most effective antibiotics for treatment. This test is generally preferred when a patient shows manifestations of a urinary tract infection, such as frequent or painful urination, cloudy or foul-smelling urine, or lower abdominal pain.Purpose of the TestThe primary goals of a urine culture and sensitivity test are to:Determine the specific bacteria causing the...
1.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Advancements in extracellular vesicle research.

Extracellular vesicle·2026
Same author

The Wnt inhibitor Tiki2 maintains articular cartilage homeostasis and protects against osteoarthritis.

Science signaling·2026
Same author

DAB2IP modulates intestinal inflammation by enhancing ILC3 function in the gut.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same author

Tiki2 restrains Wnt5a/Ror signaling to limit inflammation in acute kidney injury.

Communications biology·2026
Same author

Targeting ST3GAL1 to downregulate ligands for the glycoimmune checkpoint Siglec-7 and reverse immune escape in hepatocellular carcinoma.

Cancer immunology, immunotherapy : CII·2026
Same author

Impacts of Globo H Ceramide on Tumor Microenvironment.

Advances in experimental medicine and biology·2026

Related Experiment Video

Updated: Feb 1, 2026

Assay Development for High Content Quantification of Sod1 Mutant Protein Aggregate Formation in Living Cells
11:12

Assay Development for High Content Quantification of Sod1 Mutant Protein Aggregate Formation in Living Cells

Published on: October 4, 2017

7.6K

Development of a novel, sensitive cell-based corin assay.

Pascal Lambertz1, Laura Theisen1, Natalie Längst1

  • 1Lead Discovery Wuppertal, Bayer AG, Pharma Research and Development Center, Aprather Weg 18a, D-42096 Wuppertal, Germany.

Biochemical Pharmacology
|December 17, 2018
PubMed
Summary

Researchers developed a new fluorescence-based reporter cell line to detect corin activity, which activates atrial natriuretic peptide (ANP) for heart protection. This system enables sensitive measurement of pro-ANP to ANP conversion by corin.

Keywords:
ANP receptorAtrial natriuretic peptideCorinReporter assaySerine proteasecGMP

More Related Videos

Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications
08:23

Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications

Published on: June 23, 2016

12.8K
Highly Sensitive Assay for Measurement of Arenavirus-cell Attachment
08:34

Highly Sensitive Assay for Measurement of Arenavirus-cell Attachment

Published on: March 2, 2016

10.1K

Related Experiment Videos

Last Updated: Feb 1, 2026

Assay Development for High Content Quantification of Sod1 Mutant Protein Aggregate Formation in Living Cells
11:12

Assay Development for High Content Quantification of Sod1 Mutant Protein Aggregate Formation in Living Cells

Published on: October 4, 2017

7.6K
Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications
08:23

Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications

Published on: June 23, 2016

12.8K
Highly Sensitive Assay for Measurement of Arenavirus-cell Attachment
08:34

Highly Sensitive Assay for Measurement of Arenavirus-cell Attachment

Published on: March 2, 2016

10.1K

Area of Science:

  • Biochemistry
  • Cardiovascular Biology
  • Enzymology

Background:

  • Corin, a serine protease in cardiomyocytes, activates atrial natriuretic peptide (ANP), crucial for cardioprotection.
  • Existing ANP reporter cell lines utilize aequorin for luminescence-based detection.
  • A novel reporter system is needed for enhanced sensitivity and stability in corin activity assays.

Purpose of the Study:

  • To generate and validate a novel ANP reporter cell line using the GCaMP6 calcium biosensor.
  • To assess the utility of this new system for detecting pro-ANP to ANP conversion by corin.
  • To characterize corin activity in various cellular and purified contexts.

Main Methods:

  • Generation of a reporter cell line expressing ANP receptor, GCaMP6, and pro-ANP.
  • Assay development utilizing fluorescence-based detection of ANP-mediated calcium signaling.
  • Testing purified soluble corin (wildtype and mutant) and corin activity in transfected HEK 293 and HL-1 cells (including knockout).

Main Results:

  • The GCaMP6 reporter system produced stable, long-lasting fluorescence signals upon ANP stimulation.
  • Pro-ANP to ANP conversion was successfully detected using purified wildtype corin, but not the inactive mutant.
  • Cellular corin activity was confirmed in HEK 293 and HL-1 cells, and absent in corin knockout cells.

Conclusions:

  • The novel fluorescence-based ANP reporter cell line is highly effective for sensitive corin activity detection.
  • This system validates corin's role as the primary pro-ANP convertase.
  • The reporter cell line holds potential for identifying and characterizing new corin modulators.