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Updated: Feb 1, 2026

Evaluation of the Cell Invasion and Migration Process: A Comparison of the Video Microscope-based Scratch Wound Assay and the Boyden Chamber Assay

Evaluation of the Cell Invasion and Migration Process: A Comparison of the Video Microscope-based Scratch Wound Assay and the Boyden Chamber Assay

Published on: November 17, 2017

Is your assay stable? Using process stability and capability to evaluate assay performance.

Robert L Schmidt¹, Lauren N Pearson¹

¹Department of Pathology and ARUP Laboratories, University of Utah, Salt Lake City, UT, United States.

Clinica Chimica Acta; International Journal of Clinical Chemistry

|December 17, 2018

Summary

This summary is machine-generated.

Stability analysis offers key metrics for evaluating laboratory assay performance and identifying areas for process improvement. This method helps prioritize which assays require enhancement for better precision and capability.

Keywords:

Capability Process Improvement Quality Control Stability Statistical Process Control

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Area of Science:

Clinical Chemistry
Laboratory Medicine
Process Improvement

Background:

Laboratories strive to enhance assay precision and process capability.
Metrics are needed to evaluate performance, identify improvement potential, and prioritize projects.
Stability analysis measures freedom from extrinsic variation; capability analysis assesses meeting external requirements.

Purpose of the Study:

To demonstrate how stability analysis provides metrics for evaluating assay performance.
To show how stability analysis indicates potential for assay improvement.
To illustrate the combined use of stability and capability analyses for prioritizing assay improvement.

Main Methods:

Employed the SR test and analysis of variance (ANOVA).
Compared short-term and long-term stability for two distinct laboratory assays.
Provided detailed calculations for illustrative purposes.

Main Results:

One assay, thyroid stimulating hormone (TSH), demonstrated stability.
The second assay, methotrexate, exhibited instability.
Stability analysis yielded quantifiable metrics for performance evaluation.

Conclusions:

Stability analysis is a valuable tool for assessing laboratory assay performance.
The metrics derived from stability analysis can guide process improvement initiatives.
Prioritization of assay improvement efforts can be effectively achieved using stability and capability analyses.