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Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames
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Deep sequencing salivary proteins for periodontitis using proteomics.

Myung-Seop Shin1, Yun-Gon Kim2, Yoo Jin Shin1

  • 1Department of Preventive and Social Dentistry, School of Dentistry, Seoul National University, 101 Daehak-ro, Jongno-gu, Seoul, 03080, South Korea.

Clinical Oral Investigations
|December 17, 2018
PubMed
Summary

Saliva biomarkers S100A8 and S100A9 show promise for detecting periodontitis. A rapid test kit using these proteins could aid in early screening and promote periodontal health.

Keywords:
Ingenuity Pathway AnalysisPeriodontitisProtein biomarkerProteomicsSaliva

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Area of Science:

  • Biochemistry
  • Proteomics
  • Biomarker Discovery

Background:

  • Saliva contains proteins that can serve as indicators for periodontal diseases.
  • Periodontitis is a significant oral health concern requiring effective diagnostic tools.

Purpose of the Study:

  • To identify salivary protein biomarkers for periodontitis using mass spectrometry.
  • To validate the identified biomarkers for potential clinical application.

Main Methods:

  • Shotgun proteomics was performed on saliva samples from 207 participants.
  • Statistical analyses including principal component analysis and Ingenuity Pathway Analysis were used.
  • Enzyme-linked immunosorbent assay (ELISA) was employed for biomarker validation in a separate cohort.

Main Results:

  • Shotgun proteomics identified S100A8 and S100A9 as candidate salivary biomarkers for periodontitis.
  • ELISA confirmed significantly higher levels of S100A8 and S100A9 in individuals with periodontitis (p < 0.05).

Conclusions:

  • Salivary S100A8 and S100A9 are validated as potential biomarkers for periodontitis.
  • A rapid test kit utilizing these biomarkers could facilitate early screening and improve periodontal health outcomes.