Related Experiment Video

Updated: Feb 1, 2026

A Visual Guide to Sorting Electrophysiological Recordings Using 'SpikeSorter'
10:31

A Visual Guide to Sorting Electrophysiological Recordings Using 'SpikeSorter'

Published on: February 10, 2017

11.6K

Visualization technologies for 5-ALA-based fluorescence-guided surgeries

Linpeng Wei1, David W Roberts2,3,4,5, Nader Sanai6

  • 1Department of Mechanical Engineering, University of Washington, Seattle, WA, 98195, USA. lpwei@uw.edu.

Journal of Neuro-Oncology
|December 17, 2018
PubMed
Abstract

Insights

New visualization techniques enhance 5-aminolevulinic acid (5-ALA) fluorescence guidance for glioma surgery. These advancements improve the detection and resection of challenging low-grade and deep-seated gliomas, overcoming limitations of conventional microscopy.

Area of Science:

  • Neurosurgery
  • Oncology
  • Medical Imaging

Background:

  • 5-aminolevulinic acid (5-ALA) fluorescence-guided surgery improves malignant glioma resection.
  • Conventional wide-field microscopy struggles with low-grade, non-enhancing, and deep-seated gliomas due to low fluorescence detection.

Purpose of the Study:

  • To review limitations of current visualization technologies in 5-ALA guided glioma surgery.
  • To survey emerging visualization prototypes for improved glioma resection.
  • To offer a neurosurgical perspective on future implementation of these technologies.

Main Methods:

  • Analysis of factors limiting sensitivity and accuracy of wide-field surgical microscopy.
  • Survey of commercial and research-based visualization prototypes.
  • Evaluation of principles, advantages, disadvantages, and clinical translation status of new technologies.

Main Results:

  • High-sensitivity probe-based techniques detect PpIX in low-grade gliomas and infiltrative margins.
  • Red-light illumination enables deep-tissue PpIX imaging up to 5 mm.
  • Spectroscopic methods provide more accurate quantification of PpIX expression.

Conclusions:

  • Advanced visualization technologies significantly enhance the sensitivity and accuracy of 5-ALA fluorescence guidance.
  • Ongoing refinement of these technologies promises further improvements in glioma resection extent.
  • These innovations are crucial for optimizing outcomes in glioma patients.
Keywords:
5-ALAGliomasIntraoperative guidanceMicroscopyPpIXSpectroscopy

More Related Videos

Tissue-simulating Phantoms for Assessing Potential Near-infrared Fluorescence Imaging Applications in Breast Cancer Surgery11:05

Tissue-simulating Phantoms for Assessing Potential Near-infrared Fluorescence Imaging Applications in Breast Cancer Surgery

Published on: September 19, 2014

12.7K
Deep Fluorescence Observation in Rice Shoots via Clearing Technology07:21

Deep Fluorescence Observation in Rice Shoots via Clearing Technology

Published on: June 27, 2022

3.3K

Related Concept Videos

5-Number Summary01:04

5-Number Summary

In a dataset, the 5-number summary includes the minimum data value, the data value of the first quartile, the median data value or data value of the second quartile, the data value of the third quartile, and the maximum data value. These 5 data values can be visualized as a box and whisker plot.
In a box plot, the minimum and maximum data values represent the lower and upper whiskers in the graph, and the median is designated as the center of the box in the chart. The first quartile and third...
5.7K
Hybridoma Technology01:31

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation,...
17.7K
Health Information Technology and Healthcare Information System01:30

Health Information Technology and Healthcare Information System

Health Information Technology (HIT)
Health Information Technology, commonly called HIT, integrates advanced information systems and technology in healthcare settings. Its primary functions include:
3.4K
Chemotherapy-Induced Nausea and Vomiting: 5-HT3 Receptor Antagonists01:27

Chemotherapy-Induced Nausea and Vomiting: 5-HT3 Receptor Antagonists

5-HT3 receptor antagonists, such as dolasetron, granisetron (Kytril), ondansetron (Zofran), and palonosetron (Axoli), are crucial in managing chemotherapy-induced nausea and vomiting (CINV) and postoperative nausea. These drugs selectively block 5-HT3 receptors in the visceral vagal and spinal afferent nerves, chemoreceptor trigger zone, and the vomiting center. They have a rapid onset of action and can be given as a single dose before chemotherapy. Ondansetron and granisetron, in particular,...
666
Visual System01:26

Visual System

Light enters the eye through the cornea, a transparent, dome-shaped surface covering the surface of the eyeball that helps to direct and focus incoming light. This light is then channeled toward the pupil, an adjustable opening whose size is controlled by the iris. The iris, a pigmented muscle, regulates the amount of light entering the eye by contracting or dilating the pupil, thereby ensuring optimal light levels for clear vision.
Once through the pupil, the light passes through the lens, a...
1.8K
Visual Agnosia01:12

Visual Agnosia

Visual agnosia is a condition characterized by the inability to recognize visually presented objects despite having normal vision. For instance, a person with visual agnosia can describe the shape and color of an object but cannot identify or name it. This impairment does not affect their visual field, acuity, color vision, brightness discrimination, language, or memory. An example of this condition in a social setting is someone at a dinner party asking for "that silver thing with a round...
1.1K