Related Experiment Video

Updated: Feb 1, 2026

ROS Live Cell Imaging During Neuronal Development
09:25

ROS Live Cell Imaging During Neuronal Development

Published on: February 9, 2021

4.8K

Cyclophilin D deficiency protects against the development of mitochondrial ROS and cellular inflammation in aorta

Xiaojing Liu1, Heng Du2, Dan Chen3

  • 1Deparment of Endocrinology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong, 250021, China; Shandong Key Laboratory of Endocrinology and Lipid Metabolism, Jinan, Shandong, 250021, China; Institute of Endocrinology and Metabolism, Shandong Academy of Clinical Medicine, Jinan, Shandong, 250021, China.

Biochemical and Biophysical Research Communications
|December 18, 2018
PubMed
Abstract

Insights

Mitochondrial cyclophilin D (CypD) deficiency improves metabolic health and reduces vascular inflammation. Blocking CypD enhances resistance to inflammatory injuries in the aorta, offering a potential therapeutic target for cardiovascular disease.

Area of Science:

  • Cardiovascular Disease Research
  • Mitochondrial Biology
  • Inflammation and Immunology

Background:

  • Inflammation and oxidative stress are key drivers of cardiovascular disease pathology.
  • Mitochondrial cyclophilin D (CypD) regulates mitochondrial reactive oxygen species (ROS) generation and is linked to cellular inflammation.
  • The role of CypD in vascular inflammatory responses remains largely unexplored.

Purpose of the Study:

  • To investigate the impact of CypD deficiency on vascular inflammation under physical conditions.
  • To determine if CypD plays a role in modulating the inflammatory response within the aorta.

Main Methods:

  • Utilized CypD knockout (KO) mice and littermate controls.
  • Assessed aortic mitochondrial function, including complex III activity and ROS generation.
  • Quantified the expression of inflammatory markers (VCAM-1, IL-6, TNF-α) in mouse aortas.

Main Results:

  • CypD deficiency improved whole-body metabolic status in mice.
  • Aortic mitochondria showed increased complex III activity and reduced ROS generation in CypD KO mice.
  • Expression of key inflammatory molecules (VCAM-1, IL-6, TNF-α) was attenuated in the aorta of CypD-deficient mice.

Conclusions:

  • Mitochondrial CypD is implicated in regulating aortic inflammation.
  • Blocking CypD enhances vascular resilience against inflammatory damage.
  • Targeting mitochondrial CypD presents a potential strategy for mitigating vascular inflammatory injuries.
Keywords:
Cyclophilin DMitochondriaOxidative stressVascular inflammation

More Related Videos

Flow Cytometry Analysis of Immune Cells Within Murine Aortas15:15

Flow Cytometry Analysis of Immune Cells Within Murine Aortas

Published on: July 1, 2011

35.3K
Development of a Mobile Mitochondrial Physiology Laboratory for Measuring Mitochondrial Energetics in the Field08:54

Development of a Mobile Mitochondrial Physiology Laboratory for Measuring Mitochondrial Energetics in the Field

Published on: August 27, 2021

1.8K

Related Concept Videos

Inflammation01:38

Inflammation

Overview
62.1K
Animal Mitochondrial Genetics02:59

Animal Mitochondrial Genetics

Among all the organelles in an animal cell, only mitochondria have their own independent genomes. Animal mitochondrial DNA is a double-stranded, closed-circular molecule with around 20,000 base pairs. Mitochondrial DNA is unique in that one of its two strands, the heavy, or H, -strand is guanine rich, whereas the complementary strand is cytosine rich and called the light, or L, -strand. Compared to nuclear DNA, mitochondrial DNA has a very low percentage of non-coding regions and is marked by...
9.2K
The Aorta01:14

The Aorta

The aorta is the largest artery in the human body. It originates from the left ventricle of the heart and extends down to the abdomen, where it splits into two smaller arteries. Structurally, it can be divided into four main parts: the ascending aorta, the aortic arch, the thoracic aorta, and the abdominal aorta.
The average diameter of the aorta is approximately 2-3 cm, but the size can vary depending on the section of the aorta and the individual's age, sex, and body size. The aorta is...
2.7K
The Arch of Aorta01:10

The Arch of Aorta

The coronary arteries, originating from the ascending aorta, bifurcate from two sinuses located within the ascending aorta. Positioned just above the aortic semilunar valve, these sinuses house essential aortic baroreceptors and chemoreceptors, crucial for maintaining cardiac function. The left coronary artery and the right coronary artery branch off from the left posterior and anterior aortic sinuses, respectively.
Encircling the heart, the coronary arteries form a ring-like structure before...
1.7K
Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes02:16

Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes

The present-day mitochondrial and chloroplast genomes have retained some of the characteristics of their ancestral prokaryotes and also have acquired new attributes during their evolution within eukaryotic cells. Like prokaryotic genomes, mitochondrial and chloroplast genomes neither bind with histone-like proteins nor show complex packaging into chromosome-like structures, as observed in eukaryotes. Unlike mitotic cell divisions observed in eukaryotic cells, mitochondria and chloroplasts...
16.0K
Export of Mitochondrial and Chloroplast Genes02:19

Export of Mitochondrial and Chloroplast Genes

A eukaryotic cell can have up to three different types of genetic systems: nuclear, mitochondrial, and chloroplast. During evolution, organelles have exported many genes to the nucleus; this transfer is still ongoing in some plant species. Approximately 18% of the Arabidopsis thaliana nuclear genome is thought to be derived from the chloroplast’s cyanobacterial ancestor, and around 75% of the yeast genome derived from the mitochondria’s bacterial ancestor. This export has occurred...
4.2K