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Updated: Feb 1, 2026

Isolation of Salivary Epithelial Cells from Human Salivary Glands for In Vitro Growth as Salispheres or Monolayers
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Isolation of Salivary Epithelial Cells from Human Salivary Glands for In Vitro Growth as Salispheres or Monolayers

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Human epithelial growth factor receptor 2 in human salivary carcinoma ex pleomorphic adenoma: a potential therapeutic

Liang Xia1,2, Yang Wang1, Yuhua Hu1

  • 1Department of Oral Pathology, Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory of Stomatology, Shanghai 200011, People's Republic of China, tian0304_cn@163.com.

Cancer Management and Research
|December 18, 2018
PubMed
Abstract

Insights

Inhibiting human epithelial growth factor receptor 2 (HER2) in salivary carcinoma ex pleomorphic adenoma (CXPA) significantly reduced tumor proliferation and activated apoptosis. This suggests HER2 inhibition as a potential targeted therapy for aggressive CXPA.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Salivary carcinoma ex pleomorphic adenoma (CXPA) is a malignancy with potential for aggressive behavior.
  • Human epithelial growth factor receptor 2 (HER2) is implicated in various cancers and may play a role in CXPA progression.

Purpose of the Study:

  • To investigate the role of HER2 in CXPA.
  • To evaluate the therapeutic potential of HER2 inhibition in CXPA cell lines.

Main Methods:

  • HER2 expression and amplification were assessed in CXPA cell lines (SM-AP1, SM-AP4) using immunofluorescence and fluorescence in situ hybridization.
  • The effects of a HER2 inhibitor on cell proliferation, cell cycle, apoptosis, and downstream signaling pathways (PI3K/AKT, MAPK/ERK) were analyzed.

Main Results:

  • HER2 was found to be overexpressed and amplified in the studied CXPA cell lines.
  • HER2 inhibition led to significant induction of tumor cell proliferation and cell cycle progression.
  • Apoptosis was activated, and downstream signaling pathways PI3K/AKT and MAPK/ERK were significantly inhibited following HER2 blockade.

Conclusions:

  • Overexpression and amplification of HER2 in CXPA suggest its involvement in tumor development.
  • Targeting HER2 represents a promising novel therapeutic strategy for managing the aggressive biological behavior of CXPA.
Keywords:
HER2amplificationapoptosiscarcinoma ex pleomorphic adenomaproliferationtarget therapy

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