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TRIM14 promotes colorectal cancer cell migration and invasion through the SPHK1/STAT3 pathway

Zhonghai Jin1, Hongguang Li1, Xiaofei Hong1

  • 1Department of Gastroenterology, Yiwu Hospital, Wenzhou Medical University, 699 Jiangdong Middle Road, Yiwu, 322000 China.

Cancer Cell International
|December 18, 2018
PubMed
Abstract

Insights

Tripartite Motif Containing 14 (TRIM14) promotes colorectal cancer (CRC) cell migration and invasion. Targeting TRIM14 may offer a new strategy to prevent CRC metastasis.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Colorectal cancer (CRC) is a leading cause of cancer mortality.
  • Tripartite Motif Containing 14 (TRIM14), a TRIM family protein, is implicated in various cancers.
  • Understanding TRIM14's role in CRC is crucial for developing new therapies.

Purpose of the Study:

  • To investigate TRIM14 expression levels in colorectal cancer tissues.
  • To determine the impact of TRIM14 on colorectal cancer cell migration and invasion.
  • To elucidate the molecular mechanisms underlying TRIM14's function in CRC.

Main Methods:

  • Quantitative real-time PCR for TRIM14 mRNA expression.
  • Transwell assays to assess cell migration and invasion.
  • Western blot analysis for protein expression and signaling pathway activation.

Main Results:

  • TRIM14 expression is significantly upregulated in CRC tissues compared to non-cancerous tissues.
  • TRIM14 knockdown inhibits CRC cell migration and invasion; overexpression enhances these processes.
  • TRIM14 positively regulates Sphingosine Kinase 1 (SPHK1) and STAT3 signaling, including MMP2, MMP9, and VEGF expression.
  • Inhibition of the SPHK1/STAT3 pathway reverses TRIM14-induced CRC cell migration and invasion.

Conclusions:

  • TRIM14 plays a critical role in promoting colorectal cancer cell migration and invasion.
  • TRIM14 emerges as a potential molecular target for preventing CRC metastasis.
  • Targeting TRIM14 and its associated signaling pathways could offer novel therapeutic strategies for CRC.
Keywords:
InvasionMigrationSPHK1STAT3TRIM14

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