[Changes in various biochemical and physico-chemical properties of hemoglobin during its aging]

It is shown that hemoglobin senescence leads to both a decrease of amide groups in it and redistribution of the radioactive label (55Fe) in electrophoretic fractions. It is supposed that an increase of the radio-active label in highly mobile hemoglobin fractions of the "old" erythrocytes by 78 and 43% as compared to that of the "young" erythrocytes is related to the deamidation-mediate growth of the negative charge of this protein molecule or partial denaturation of its quaternary structure. The resistance of hemoglobin of the "old" erythrocytes is shown to decrease to acid tissue proteinases of the lysosomal fraction as against hemoglobin of the "young" erythrocytes.