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Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages
10:07

Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages

Published on: June 23, 2013

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A simple method to increase the proportion of bone marrow-derived macrophages positive for M-CSFR using the reducing

Ryota Hashimoto1, Hiroyuki Daida2, Takao Okada1

  • 1Department of Physiology, Juntendo University Faculty of Medicine, Hongo 2-1-1, Bunkyo-ku, Tokyo, 113-8421, Japan.

Methodsx
|December 18, 2018
PubMed

Insights

A new method using dithiothreitol (DTT) increases the proportion of bone marrow-derived macrophages (BM-MΦ) expressing macrophage colony-stimulating factor receptor (M-CSFR). This DTT treatment allows for better investigation into M-CSFR

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Macrophage colony-stimulating factor receptor (M-CSFR) plays a role in macrophage function.
  • A low proportion of M-CSFR expression on bone marrow-derived macrophages (BM-MΦ) hinders research.

Purpose of the Study:

  • To develop a method for increasing M-CSFR positive BM-MΦ.
  • To investigate M-CSFR regulation in BM-MΦ.

Main Methods:

  • Primary mouse BM-MΦ were treated with dithiothreitol (DTT).
  • M-CSFR expression was analyzed using flow cytometry.
  • DTT-treated BM-MΦ were subsequently treated with lipopolysaccharide (LPS).

Main Results:

  • Dithiothreitol (DTT) treatment significantly increased the proportion of M-CSFR positive BM-MΦ in a time-dependent manner.
  • Lipopolysaccharide (LPS) treatment abrogated the DTT-induced upregulation of M-CSFR.
  • The proportion of M-CSFR positive BM-MΦ approximately doubled after DTT treatment.

Conclusions:

  • DTT treatment offers a simple and effective method to enhance M-CSFR expression on BM-MΦ.
  • This method facilitates research into the role of M-CSFR downregulation in disease pathogenesis.
Keywords:
AtherosclerosisBone marrow-derived macrophagesDithiothreitol (DTT)Lipopolysaccharide (LPS)Macrophage colony-stimulating factor receptor (M-CSFR)Method to increase the proportion of macrophages positive for M-CSFR

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