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Transcriptomic data for analyzing global gene expression patterns in Methicillin-resistance Staphylococcus aureus in

Shrikant Pawar1,2, Xiangyu Yao3, ChungDar Lu4

  • 1Department of Biology, Georgia State University, 33 Gilmer Street SE, 30303 Atlanta, GA, USA.

Data in Brief
|December 18, 2018
PubMed

Insights

Methicillin-resistant Staphylococcus aureus (MRSA) resistance to antibiotics can be altered by spermine. A specific MRSA mutation abolishes spermine-beta-lactam synergy, offering new insights into bacterial resistance mechanisms.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Methicillin-resistant Staphylococcus aureus (MRSA) is a significant pathogen resistant to beta-lactam antibiotics due to altered penicillin-binding protein (PBP2a).
  • Exogenous spermine has demonstrated dose-dependent inhibitory effects on the growth of various bacteria, including Staphylococcus aureus.

Purpose of the Study:

  • To investigate the impact of a PBP2 gene mutation on spermine resistance and spermine-beta-lactam synergy in MRSA.
  • To elucidate the molecular mechanisms underlying spermine-beta-lactam synergy sensitization in MRSA through transcriptomic analysis.

Main Methods:

  • Selection of an MRSA Mu50 derivative (MuM) with a PBP2 gene mutation conferring spermine resistance.
  • Transcriptomic profiling comparing MuM and wild-type Mu50 under various conditions: no treatment, high-dose spermine exposure, and combined spermine-beta-lactam treatment.

Main Results:

  • The PBP2 mutation in MuM resulted in spermine resistance and abolished the synergistic effect of spermine and beta-lactams.
  • Transcriptomic data provided insights into the differential gene expression patterns in response to spermine and beta-lactam treatments in wild-type and mutant MRSA strains.

Conclusions:

  • The PBP2 gene mutation significantly alters MRSA's response to spermine and beta-lactam antibiotics.
  • Understanding these mechanisms can inform the development of novel therapeutic strategies against MRSA infections.

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